一株中度嗜盐细菌whb45的鉴定及其抗菌.docVIP

一株中度嗜盐细菌whb45的鉴定及其抗菌与抗肿瘤活性筛选 陈雷1,2,3 王光玉2 卜同1,3 张允斌2 刘明1 张军4 林秀坤1* (1. 中国科学院海洋研究所实验海洋生物学重点实验室 山东 青岛 266071)(2. 哈尔滨工业大学(威海)海洋学院 山东 威海 264209)(3. 中国科学院研究生院 北京 100039)(4. 山东省医药工业设计院 山东 济南 250013) 摘 要: 从盐场中分离鉴定中度嗜盐细菌并对其潜在的抗菌和抗肿瘤活性进行评价。从山东威海的鹿道口盐场分离嗜盐细菌, 对菌株whb45进行形态学和生理生化特性研究, 测定其16S rRNA序列并通过同源性比对进行系统发育分析, 采用抗菌和细胞毒模型进行活性筛选。试验结果表明, 菌株whb45为中度嗜盐细菌, whb45与Halobacillus trueperi在形态和生理生化特征方面最接近, 16S rRNA序列相似性为99%。whb45的粗提物对多种细菌、真菌和肿瘤细胞的生长都具有较强的抑制作用, 可以作为发现生物活性物质的潜在的新来源。 关键词: 中度嗜盐细菌, 鉴定, 喜盐芽孢杆菌属, 抗菌活性, 抗肿瘤活性 Identification of a Moderately Halophilic Bacterium whb45and Screening of Its Antimicrobial and Antitumor Activity CHEN Lei1,2,3 WANG Guang-Yu2 BU Tong1,3 ZHANG Yun-Bin2 LIU Ming1ZHANG Jun4 LIN Xiu-Kun1* (1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China)(2. College of Marine Science, Harbin Institute of Technology at Weihai, Weihai, Shandong 264209, China)(3. Graduate School, Chinese Academy of Sciences, Beijing 100039, China)(4. Shandong Province Design Institute of Pharmaceutical Industry, Jinan, Shandong 250013, China) Abstract: The aim of this study was to isolate and identify moderately halophilic bacteria from solar saltern and to investigate their potential antimicrobial and antitumor activities. Halophilic bacteria were isolated from Ludaokou Solar Saltern in Weihai of Shandong Province. The identification of the strain whb45 was determined by their morphological characteristics, physiological and biochemical tests and phylogenetic analysis based on 16S rRNA sequence comparison. The antimicrobial activity and cytotoxic activity were screened. Our results showed that the strain whb45 was belonged to moderately halophilic bacterium. Phylogenetic analysis showed the strain whb45 had a 99% homology with Halobacillus trueperi. The crude extract from whb45 showed stronger antimicrobial activities which could inhibit the growth of several bacteria and fungi