采取气相色谱-质谱法分析药对麻黄-羌活的挥发油.pdfVIP

第 38 卷第 5 期 中南大学学报( 自然科学版) Vol.38 No.5 2007 年 10 月 J. Cent. South Univ. (Science and Technology) Oct. 2007 采用气相色谱−质谱法分析药对麻黄−羌活的挥发油 1, 2 1 1 1 李国辉 ，李晓如 ，邹 桥 ，谭斌斌 (1. 中南大学 化学化工学院，湖南 长沙，410083； 2. 湖南城市学院 化学与环境工程系，湖南 益阳，413000) 摘 要：采用气相色谱−质谱(GC−MS)法分离测定了药对麻黄−羌活、单味药麻黄和羌活的挥发油，并对其重叠色 谱峰采用化学计量学解析法进行了分辨，得到药对和各单味药的纯色谱曲线和质谱。药对麻黄−羌活、单味药麻 黄和羌活分辨出的色谱峰，通过质谱库对其进行定性，分别得到 58，51 和 52 个定性结果，占总含量的 83.75%, 88.70% 和 80.79%。实验结果表明：药对麻黄−羌活中挥发油化学成分有 40 种来自羌活，13 种来自麻黄，新产生 了 10 种化合物。 关键词：药对麻黄−羌活；挥发油；气相色谱−质谱；化学计量学解析法 中图分类号：O657 文献标识码：A 文章编号：1672-7207(2007)05−0888−05 Determination of volatile components in Herba Ephedrae−Rhizoma seu Radix Notopeterygii by gas chromatography−mass spectrometry 1, 2 1 1 1 LI Guo-hui , LI Xiao-ru , ZOU Qiao , TAN Bin-bin (1. School of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China; 2. Department of Chemistry and Environment Engineering, Hunan City College, Yiyang 413000, China) Abstract: Determination of volatile components in drug pair (DP) Herba Ephedrae−Rhizoma seu Radix Notopeterygii (HE-RsRN), single drug HE and RsRN was performed using two-dimensional gas chromatography−mass spectrometry (GC-MS) data coupled with chemometric resolution method (CRM). With the help of CRM, the two-dimensional data obtained from GC-MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound. Using