分子生物学(中英对照有备注)7.pptVIP

* Mechanisms of translation regulation also exists at target specific mRNAs or groups of mRNAs, rather than all transcripts. This is known as mRNA-specific control, and it depends very much on sequences within individual mRNA transcripts. 翻译调控机理也存在于对特殊的目标mRNA分子或目标mRNA群的调控，而不仅仅用于调控所有的转录产物。这就是mRNA特异性控制，它在很大程度上依赖于单个mRNA转录产物内部的序列。 * For example, RNAs with a uracil-rich region called a CPE can be regulated by a mechanism very similar to the one just described for global control. 例如，具有称为CPE的富含尿嘧啶区域的RNA可以被一种我们刚刚描述过的与全局控制非常相似的机理所调控。 * The CPE region binds to a protein, CPEB. CPEB subsequently attracts a protein called Maskin. Maskin acts like a 4E-BP, binding to eIF4E and preventing its association with eIF4G. As a consequence, mRNAs with the CPE sequence have greater difficulty forming a complex with a ribosome, and less protein is made from these mRNAs. CPE区域与CPEB蛋白结合，CPEB紧接着吸引一种称为掩蔽蛋白的蛋白质。掩蔽蛋白的作用类似于4E-BP，它能与eIF4E结合从而防止eIF4E与eIF4G的结合。结果，具有CPE序列的mRNA与核糖体形成复合体的难度大大增加，从它们那儿产生的蛋白质自然也就少了。 * Another example of mRNA specific control relates to iron metabolism. Ferritin is a kind of protein used to bind irons in the cell. When iron levels in the cell are high, ferritin is used to bind and sequester iron from the cytoplasm. When iron levels are low, the activity of ferritin is counterproductive. Thermotoga Maritima (a kind of bacteria) 另一个mRNA特异性控制的例子与铁代谢有关，在细胞中铁蛋白是一种用来与铁结合的蛋白质。当细胞中铁的含量高时，铁蛋白被用来从细胞质中结合并隔离铁。当铁含量低时，铁蛋白的作用正好相反。Thermotoga Maritima（一种细菌） * As in the regulation of transferrin, another iron-related protein discussed above, aconitase is used to regulate ferritin levels. However, regulation of ferritin occurs at the level of translation, not mRNA stability. When iron concentration is low, aconitase binds to the 5’-UTR of ferritin mRNA and inhibits translation, physically preventing the association of mRNA with ribosomal subunits. By contrast, when iron is abundant, aconitase does not bind to ferritin mRNA and translation proceeds normally. 与上面讨论过的另一个铁相关蛋白（运铁蛋白）