2型糖尿病大鼠心肌中HGF的表达及替米沙坦的干预作用.docVIP

替米沙坦对糖尿病大鼠肝细胞生长因子的影响 王小华，殷士良，赵玉萍，刘晓峰，赵春艳，周凤娟 河北省唐山市滦县人民医院 内分泌科 河北 滦县 063700 摘要: 目的 探讨替米沙坦对糖尿病大鼠肝细胞生长因子的影响。方法 雄性SD大鼠40只,单纯随机分为：正常组(C组), 糖尿病模型组(D组),替米沙坦干预组(T组)。D和T组高糖高脂饲料喂养4周后以25mg／kg链脲佐菌素（STZ）尾静脉一次性注射，C组常规饲料喂养4周后以等剂量的枸橼酸盐尾静脉注射。给予STZ后一周， T组给予替米沙坦8.34 mg／kg/d，C和D组等剂量温水灌胃。所有大鼠继续原饲料喂养，给药l2周末结束实验。双抗体夹心ELISA法和western blot法分别检测血清和心肌中HGF的表达。结果 血清和心肌中HGF水平D组，T组高于C组 ，T组高于D组。结论 替米沙坦能够增加糖尿病大鼠HGF的表达。 关键词：2型糖尿病；心肌；肝细胞生长因子；替米沙坦 Effects of telmisartan to Expression of hepatocyte growth factor in type 2 diabetic rats Wangxiaohua, Yinshiliang, Zhaoyuping, Liuxiaofeng, Zhaochunyan, Zhoufengjuan Dept. of Endocrinology, People Hospital, Luan County，Tangshan CityHebei Province, Luanxian 063700, China Abstract: Objective To explore the effects of telmisartan to hepatocyte growth factor (HGF) in type 2 diabetic rats. Method Forty male SD rats were randomly divided into the normal group (C group), diabetes model group (D group), telmisartan intervention group (T group). Rats of D and T groups were injected by the tail vein with streptozotocin(STZ) 25mg/kg after 4- week fed with high calorie diet; C group were injected by the tail vein with the same volume citric acid buffer after 4- week fed with common diet. After 1 week injected the STZ, rats of T group were given with telmisartan 8.34 mg/kg/d and rats of C, D groups with the same volume water. The experiment ended until l2 weeks given telmisartan. Blood samples were obtained to measure the levels of HGF by double-antibody sandwich ELISA method, The semiquantitative expressions of HGF protein of myocardium were determined by western blot method. Results The levels of HGF of serum and myocardium of D, T groups were evated statisically significant compared wiht C group; The levels of HGF of T groups were evated statisically compared wiht D group. Conclution Telmisartan can increate expression of HGF in type 2 diabetic rats. Key words： Type 2 diabetes mellitus; Myocardium; Hepatocyte growth factor; Telmi