在安全输血中应用丙型肝炎病毒核心抗原检测技术的初步探索.DOCVIP

临床医学论文-在安全输血中应用丙型肝炎病毒核心抗原检测技术的初步探索 ?????????? 作者：姚仁南，张建辉，黄晓静，杨钦，曹琴，江学成 【摘要】? 本研究探讨用丙型肝炎病毒(HCV)核心抗原酶联免疫吸附技术(HCV-cAg ELISA)筛查献血员感染HCV的可行性。 对我医院2003年1月-2005年12月间的8 677份献血者血清标本进行抗-HCV初检和复检。将仅初检阳性的15份血清标本和仅复检阳性的14份血清标本，分别再做HCV-cAg ELISA和HCV RT-PCR检测。结果表明：经HCV-cAg ELISA检测，29份仅初检（15份）和仅复检（14份）抗-HCV阳性血清标本中只有5份结果为阳性，阳性率为17.24%。经HCV RT-PCR检测，29份仅初检和仅复检抗-HCV阳性血清标本中同样也只有5份阳性结果，阳性率也为17.24%。结论：HCV-cAg ELISA检测技术的敏感性与HCV RT-PCR技术类似，但成本明显降低，有可能作为HCV感染的辅助确证试验，或作为抗-HCV检测技术的更新换代技术用于安全输血中献血者血液的筛查。 【关键词】? 丙型肝炎病毒 　　Technique of Detection of Hepatitis C Core Antigen Used in? Safety? Blood Transfusion 　　　Abstract　This study was purposed to investigate the feasibility to screen donor with HCV infection by means of? HCV-cAg ELISA.? The first and repeat assays were performed for detection of serum anti-HCV in 8677 donor′ s serum specimens from January 2003 to December 2005. All serum anti-HCV speciments with positive anti-HCV from first and repeat assays were finally identified by using HCV-cAg ELISA and HCV RT-PCR methods. The results showed that only 5 serum speciments were positive anti-HCV?? by HCV-cAg ELISA?? identification? in 29 speciments including? 15 speciments with positive ant-HCV in first assays and 14 speciments with positive anti-HCV in repeat assays，the positive rate detected by HCV cAg ELISA was 17.24%. 5 serum speciments were? positive anti-HCV? by HCV RT-PCR detection also in 29 speciments mentioned above，the positive rate detected by HCV RT-PCR was 17.24% too. It is concluded that sensitivity of HCVcAg ELISA is similar to HCV RT-PCR and may be useful for the early diagnosis of hepatitic C? or? used as? a reliable method to screen donor with HCV infection in blood transfusion medicine. 　　Key words hepatitis C virus; hepatitis C virus core antigen; HCV-cAg ELISA; HCV RT-PCR ??? 目前临床上主要用抗-HCV ELISA技术筛检献血者的HCV感染状况。尽管使用的抗-HCV ELISA试剂盒均为国家“批批检”合格产品，但由于厂家之间使用HCV基因重组抗原质量及各抗原片段包被比例的不同，各厂家试剂间的灵敏度和特异性存在着一定差异，从而导致抗-HCV检测结果不一致，易产生假阳性或漏检等情况。我们从2003年1月-2005年12月采用两家厂商生产的抗-HCV ELISA试剂对867