Dickkopf-1 与抗酒石酸酸性磷酸酶5b (TRAP5b) 在类风湿关节炎骨破坏中的意义.pdfVIP

中国科技论文在线 Dickkopf-1 与抗酒石酸酸性磷酸酶 5b(TRAP5b)在类风湿关节炎骨破坏中的意 义 5 李春，马晓旭，郑莉，左瑜，杜燕，张学武** （北京大学人民医院风湿免疫科） 摘要：目的：研究类风湿关节炎(RA)患者外周血及关节液Dickkopf-1(DKK-1)水平和血清抗 酒石酸酸性磷酸酶 5b(TRAP5b)活性在骨破坏及骨代谢失衡中的意义。方法：采用酶联免疫 10 吸附法(ELISA)检测230 例RA 患者、50 例骨关节炎(OA)患者及150 例健康对照血清DKK-1 水平及和关节液 DKK-1 的浓度及血清TRAP5b 的活性浓度，比较各组DKK-1 及 TRAP5b 水平及其与临床相关指标的相关性。结果：(1) RA 患者血清DKK-1 水平明显高于健康对照 组(P0.001)和OA 患者组(P0.001)。(2) RA 关节液DKK-1 水平明显高于OA(P=0.007) ，15 例配对血清和关节液DKK-1 水平呈显著正相关(r=0.860, P0.0001)。(3) RA 骨质疏松组血清 15 TRAP5b 活性显著高于非骨质疏松组 (P0.001)。 (4) RA 患者外周血DKK-1 水平及TRA5b 活性均与病程及Sharp 评分正相关(P0.05)。结论：RA 患者外周血DKK-1 和TRAP5b 与局 部骨破坏密切相关，可作为骨破坏及骨代谢失衡的评价指标。 关键词：关节炎，类风湿；Dickkopf 1 蛋白；抗酒石酸酸性磷酸酶5b；骨质破坏 中图分类号：R593.22 20 Significance of circulating Dickkopf-1 and tartrate-resistant acid phosphatase 5b (TRAP5b) in bone destruction of patients with rheumatoid arthritis LI Chun, MA Xiaoxu, ZHENG Li, ZUO Yu, DU Yan, ZHANG Xuewu 25 (Department of Rheumatology and Immunology, Peking Unviersity Peoples Hospital) Abstract: Objectives To investigate the roles of circulating Dickkopf-1(DKK-1) and tartrate-resistant acid phosphatase 5b (TRAP5b) in RA patients with bone destruction and osteoporosis. Methods Serum samples were obtained from 230 RA, 50 osteoarthritis (OA) and 150 health controls. Synovial fluid (SF) was collected from 45 RA and 15 OA patients, and 15 paired 30 sera were collected from these RA patients. The level of DKK-1 and TRAP5b were measured by ELISA. Results Serum DKK-1 of RA patients were significantly higher than OA and health controls (P0.001); SF DKK-1 was significantly higher in RA than in OA (P=