血管紧张素II对成人脂肪间充质干细胞向心肌细胞分化的影响.DOCVIP

临床医学论文-血管紧张素II对成人脂肪间充质干细胞向心肌细胞分化的影响 【摘要】? 目的： 观察血管紧张素Ⅱ（AngⅡ）对成人脂肪间充质干细胞(ADMSCs)向心肌细胞方向分化的诱导作用，并与5氮杂胞苷（5aza）作比较. 方法： 自成人脂肪组织中分离培养ADMSCs，分别用AngⅡ（Ⅰ组）和5aza（Ⅱ组）诱导ADMSCs，同时设立对照组（Ⅲ组），光镜下观察细胞的生长情况及形态学变化，电镜下观察细胞的超微结构，免疫细胞化学方法检测心肌特异性肌钙蛋白Ⅰ（cTnⅠ），MTT法检测细胞活性，流式细胞术分析细胞周期及凋亡. 结果： Ⅰ,Ⅱ组ADMSCs诱导后呈现类似心肌细胞的形态学特征，第28日Ⅰ, Ⅱ组均表达cTnⅠ，两组间诱导转化率无差别，其中Ⅰ组ADMSCs增殖迅速，较少凋亡细胞. 结论： AngⅡ在促进ADMSCs增殖的同时诱导其向心肌细胞分化，优于传统的化学诱导剂5aza. 【关键词】? 干细胞；脂肪组织；血管紧张素Ⅱ；5氮杂胞苷；心肌细胞；诱导分化 　　【Abstract】 AIM: To study the induction effect of angiotensin Ⅱ (AngⅡ) on the differentiation of adult adiposederived mesenchymal stem cells (ADMSCs) into myocardial cells, and to compare its effect with 5azacytidine (5aza). METHODS: ADMSCs were isolated and cultured from adult adipose tissue. AngⅡ (Group Ⅰ) and 5aza (Group Ⅱ) were added to induce ADMSCs, while control group (Group Ⅲ) was set up. Cell growth and morphological changes were observed with light microscope. Electron microscope was used to observe cell ultrastructure, while immunocytochemical methods was adopted to test cardiacspecific troponinⅠ (cTnⅠ). MTT was used to assay cell viability and flow cytometry was to analyze cell cycle and apoptosis. RESULTS: ADMSCs in Group Ⅰ and Group Ⅱ showed similar myocardial cells in morphological features. Twentyeight days after induction, ADMSCs in Group Ⅰ and Group II both expressed cTnⅠ, and induced conversion rate in these 2 groups had no discrimination. ADMSCs in Group Ⅰ had higher proliferation rate and lower apoptosis rate than those of Group Ⅱ. CONCLUSION: AngⅡ can not only promote ADMSCs proliferation but also induce differentiation to the direction of myocardial cells. Its effects is better than that of traditional chemical inducer 5aza.　　　　【Keywords】 stem cells; adipose tissue; angiotensin Ⅱ; 5azacytidine; myocardial cells; induced differentiation 　　0? 引言??? 　　近年有实验证明，血管紧张素Ⅱ（angiotensin Ⅱ，AngⅡ）能够诱导人骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMMSCs）分化为心肌样细胞. 而脂肪间充质干细胞(adiposederived mesenchymal stem cell