胰岛素样生长因子结合蛋白相关蛋白1在活化肝星状细胞中的表达及意义.DOCVIP

临床医学论文-胰岛素样生长因子结合蛋白相关蛋白1在活化肝星状细胞中的表达及意义 ????????? 作者：刘立新 张骞骞 郭晓红 赵嘉慧 韩德五? 【摘要】? 目的 研究胰岛素样生长因子结合蛋白相关蛋白1(insulinlike growth factor binding proteinrelated protein1，IGFBPrP1)在转化生长因子β1(tronstorming growth factor β1,TGFβ1)诱导的肝星状细胞中表达的变化及抗IGFBPrP1抗体对TGFβ1诱导的肝星状细胞产生Ⅰ型胶原的影响。方法 大鼠肝星状细胞株(HSCT6)体外培养，分别设空白对照组(加入等量PBS)、不同浓度的TGFβ1处理组、不同浓度的抗IGFBPrP1抗体处理组，处理因素作用24 h，采用免疫组织化学染色、Western blot检测IGFBPrP1在HSCT6中的表达，ELISA检测Ⅰ型胶原的含量并进行IGFBPrP1与Ⅰ型胶原的相关性分析。结果 TGFβ1各处理组IGFBPrP1的表达均较空白对照组显著增强。抗IGFBPrP1抗体可以拮抗TGFβ1诱导的HSCT6产生的过多Ⅰ型胶原。IGFBPrP1与Ⅰ型胶原呈正相关(r=0.833，P0.01)。结论 IGFBPrP1参与了肝纤维化的形成，且可能在肝纤维化的发生、发展中占有重要地位。 【关键词】? 胰岛素样生长因子结合蛋白相关蛋白1； 肝星状细胞； 转化生长因子β1； Ⅰ型胶原 ??? 【Abstract】? Objective? To study the expression of insulinlike growth factor binding proteinrelated protein1 (IGFBPrP1) in TGFβ1 induced hepatic stellate cell and the effects of antiIGFBPrP1 antibody on the collagen Ⅰ production of TGFβ1 induced hepatic stellate cell. Methods?? Rat hepatic stellate cell line (HSCT6) was cultured in vitro, and then was incubated with phosphate buffered solution (blank control group), TGFβ1? at 2 μg/L or 4 μg/L? (TGFβ1 group), antiIGFBPrP1 antibody at 0.1 μg/L, 1 μg/L or 2 μg/L (antiIGFBPrP1 antibody group) or both (TGFβ1+antiIGFBPrP1 antibody group). Twentyfour hours later, the expression of IGFBPrP1 in HSCT6 was detected by immunohistochemical staining and western blotting; the content of collagen Ⅰ in culture supernatant of HSCT6 was measured by ELISA; the relationship between IGFBPrP1 and collagen Ⅰ was analyzed. Results? The expression of IGFBPrP1 in TGFβ1 group was significantly higher than that in blank control group. The result of ELISA showed that antiIGFBPrP1 antibody antagonized the overproduction of collagen Ⅰ by TGFβ1 induced HSCT6. There was a positive correlation between IGFBPrP1 and collagen Ⅰ (r=0.833, P0.01). Conclusions? IGFBPrP1 may play an important role in the formation and development of liver fibrosis. ??? 【Key words】? IGFBPrP1;? Hepatic stellate