选择性脑灌注对深低温停循环脑组织兴奋性氨基酸含量的影响.DOCVIP

临床医学论文-选择性脑灌注对深低温停循环脑组织兴奋性氨基酸含量的影响 ????????????????? 作者：高国栋, 李明,龙村,田良鑫, 郑军,孙立忠 【摘要】? 目的观察选择性脑灌注（SCP）对深低温停循环（DHCA）兔脑组织兴奋性氨基酸（EAA）含量的影响。方法健康大耳白兔16只，随机分为2组，每组8只：对照组（DHCA组）：DHCA 60 min；实验组（SCP组）：DHCA 60 min + 经右锁骨下动脉顺行性选择性脑灌注。每组均经体外循环降温至鼻咽温18℃，停循环，实验组停循环期间经右锁骨下动脉SCP。DHCA 60 min,复温,停机,取脑组织。氨基酸自动分析仪检测脑皮质中的谷氨酸（Glu）和天冬氨酸（Asp）的含量。结果实验组脑组织Glu含量明显高于对照组 (137.3±23.6)vs (87.0±15.9)μg/100 mg (P0.01)。结论选择性脑灌注可抑制EAA的释放，可能是其脑保护机制之一。 【关键词】? 选择性脑灌注；深低温停循环；兴奋性氨基酸 基金项目： 国家自然科学基金资助项目 　　Effects of Selective Cerebral Perfusion on the Contents of the? Excitatory Amino Acid in the Brain Tissue of Rabbits Induced by Deep Hypothermic Circulatory Arrest 　　Abstract: OBJECTIVE To observe the effects of antegrade? selective cerebral perfusion(SCP) via right subclavian artery on the contents of the excitatory amino acid (EAA) in the brain tissue of rabbits induced by deep hypothermic circulatory arrest (DHCA). METHODSSixteen rabbits weighting?? from2.3 to 2.8 kg were randomly allocated to 2 groups, each group? consists of eight rabbits. One group? served as control: received DHCA only. The other group animals? served as experiment? group: received DHCA and antegrade? selective cerebral perfusion . Each group were placed on extracorporeal circulation（ECC） and cooled to 18℃.? The period of DHCA was 60 minters. While DHCA, The experiment? group received SCP via right subclavian artery. After 60 minters of DHCA, animals were rewarmed and weaned from ECC. All animals were executed at the end of experiment and brain was removed to measure the content of EAA in the brain tissue. RESULTSIn experiment group , the content of Glu increased significantly as compared with that in control group? （137.3±23.6 μg/100mg vs? 87.0±15.9 μg/100 mg P0.01）In experiment group there were no significantly change of Asp compared with that in control group . CONCLUSIONantegrade? selective cerebral perfusion inhibits the release of EAA during DHCA in rabbits. This could be one of the mechanisms of antegrade? selective cerebral