三个水稻逆转座子相关基因的逆境响应特征_徐玲.docVIP

植物病理学报 ACTA PHYTOPATHOLOGICA SINICA 44（2）： 147-155（2014）  三个水稻逆转座子相关基因的逆境响应特征  徐 玲  1，2  ，杨 静1，刘 林1，李成云1 * （ 1 云南农业大学农业生物多样性与病虫害控制教育部重点实验室，昆明 650201；2 云南省保山学院，保山 678000） 摘要：逆转座子是植物基因组的重要成分，对基因组的大小、结构、功能和进化都有重要影响。研究表明多种生物和非生物 逆境胁迫可以激活植物逆转座子的转录，但其调控机制和功能还属未知。本研究从稻瘟菌侵染后云南地方水稻品种月亮 谷的基因转录谱中筛选出 3 个逆转座子相关基因，通过 ＲT-PCＲ 分析了逆境胁迫处理对其表达水平的影响。结果表明，稻 瘟病菌、水杨酸、2，4-D 和 NaCl 处理水稻苗都能诱导这 3 个基因的快速转录，表明这 3 个基因能够同时响应生物逆境和非 生物逆境，是研究该类逆转座子表达调控的良好候选基因。通过生物信息学分析还发现，它们在月亮谷基因组中没有发生 大的结构变异；且在水稻基因组中都具有与植物抗病相关的旁系同源物，意味着其表达后可能对抗性产生影响，因此，这些 基因在逆境和非生物逆境中的表达模式和功能值得关注。 关键词：水稻；逆转座子相关基因；逆境响应；转录；同源性  Stress responses of three retrotransposon-related genes in rice  XU Ling  1，2  ，YANG 1 1 1 1  China，Yunnan Agricultural University，Kunming 650201，China； 2 Baoshan College，Baoshan 678000，China） Abstract： Ｒetrotransposons are critical components of plant genome and have vital impact on the size，struc- ture，function and the evolution of plant genome． Studies have shown that the transcription of retrotransposons could be activated by biotic and abiotic stresses，but their regulation mechanism and function are unknown． Three retrotransposon-related genes were selected based on the microarray results of Yunnan landrace rice variety Acuche inoculated with Magnaporthe oryza and the impact of different stresses on their expression levels were analyzed by ＲT-PCＲ． The results showed that all the ways of M． oryza，SA，2，4-D and high-salt could induce the quick expression of the genes，suggesting that they could respond to both biotic and abiotic stresses and that they were ideal candidates for investigating expression and regulation of retrotransposons to stresses． Bioinforma- tics analysis showed that the 3 genes had conserved structure in Acuche and reference genome； their paralogs were related to disease-resistant． These results implied that their expression probably contributed to resistance， their expression pattern and functions in biotic and abiotic stresses were valuable to investi