高效液相色谱法定量分析固态发酵食醋中有机酸的方法优化.pdf

※分析检测 食品科学 2014, Vol.35, No.04 55 高效液相色谱法定量分析固态发酵食醋中 有机酸的方法优化 1 1 1,2 1,3 1,2,3,* 余永建 ，邓晓阳，陆震鸣 ，史劲松 ，许正宏 （1.江南大学药学院，江苏 无锡 214122 ；2. 中国科学院天津工业生物技术研究所 天津市工业生物系统与过程 工程重点实验室，天津 300308 ；3. 国家固态酿造工程技术研究中心，四川 泸州 646000 ） 摘  要：优化固态发酵食醋中9 种有机酸的高效液相色谱定量分析方法。色谱条件：以C18色谱柱进行分离，流 动相为NaH PO 溶液，流速为0.9 mL/min ，检测波长为210 nm ，可在14 min内完成检测。在优化的色谱条件下， 2 4 9 种有机酸的检测线性范围较宽，最低检出限为0.02～1.79 mg/L，加标回收率为96.02%～104.55%，相对标准偏差 0.46%～3.52% 。该方法具有灵敏、准确、稳定等优点，可用于固态发酵食醋中有机酸的测定。 关键词：固态发酵食醋；高效液相色谱；有机酸 An Optimized Method for the Analysis of Organic Acids in Solid-State Fermented Vinegars by High Performance Liquid Chromatography 1 1 1,2 1,3 1,2,3,* YU Yong-jian , DENG Xiao-yang , LU Zhen-ming , SHI Jin-song , XU Zheng-hong (1. School of Pharmaceutical Science, Jiangnan University, Wuxi 214122, China; 2. Tianjin Key Laboratory for Industrial Biological Systems and Bioprocessing Engineering, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China; 3. National Engineering Research Center of Solid-State Brewing, Luzhou 646000, China) Abstract: Quantitative determination of 9 organic acids in traditional Chinese vinegars by high performance liquid chromatography (HPLC) was optimized. The procedure of HPLC analysis was carried out on a C18 column using sodium dihydrogen phosphate as the mobile phase. The flow rate was 0.9 mL/min and the detection wavelength was 210 nm. The HPLC analysis was completed within 14 min. The linear ranges for 9 types of organic acids were wide and the l