利用脂肪酸生物标记法（PLFAs）分析水稻根际系土壤微生物.docVIP

利用脂肪酸生物标记法（PLFAs）分析水稻根系土壤微生物多样性( 刘波1，胡桂萍1，郑雪芳1，张建福2，谢华安3* (1福建省农业科学院农业生物资源研究所，福州市，350003；2福建省农业科学院水稻研究所 福州市，350018；3国家水稻改良中心福州分中心 福州市，350018) 摘要：【目的】通过分析水稻根系土壤微生物群落结构，探讨其与水稻品种特性的内在联系，为水稻育种和品种改良提供科学依据。【方法】采用脂肪酸生物标记（PLFAs）法，分析4个Ⅱ优为母本的水稻品种和3个优制5为父本的水稻品种在同等栽培条件下水稻根系微生物群落结构。【结果】试验共检测到脂肪酸生物标记（PLFAs）38条，完全分布的生物标记有23条，不完全分布的有15条；水稻根系土壤中细菌的脂肪酸生物标记含量真菌放线菌；对根系土壤特征脂肪酸生物标记16:0（细菌）、18:1ω9с（真菌）、10Me l7:0（放线菌）、10Me 16:0（硫酸盐还原细菌）和16:1ω5с（甲烷氧化菌）比较可知，优制5为父本的品种含量高，最高的为金生优制5，Ⅱ优为母本的低，含量最低的为Ⅱ优797；对PLFAs总量和水稻生育特征进行相关分析得到，PLFAs与植株有效穗和产量呈正相关，但与株高呈负相关；对水稻根系土壤脂肪酸生物量总量进行聚类发现，Ⅱ优为母本的品种聚为一类，优制5为父本的聚为一类，表现出水稻遗传特性在其根系微生物脂肪酸生物标记（PLFAs）上的差异性。【结论】水稻根系土壤微生物群落结构丰富，根系土壤微生物群落结构与品种特性有相关性，同时受水稻遗传亲缘关系远近的影响。 关键词：水稻；根系微生物；磷脂脂肪酸；多样性 Microbial community diversity analysis in the rhizosphere of rice using Phospholipid Fatty Acid biomarkers detection LIU Bo1，HU Gui－ping1，ZHENG Xue－fang1，ZHANG Jian－fu2，XIE Hua－an3* (1 Agricultural Biological Resource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003; 2 Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350018;3 Fuzhou Branch, National Rice Improvement Center of China, Fuzhou 350018) Abstract:【Objective】The goal of the research was to analyze the genetic relationship between rhizosphere microbial community structure and variety of rice by using Phospholipid Fatty Acid biomarkers detection. 【Method】The rhizosphere microbial community of rice were sampled to analyzed by Phospholipid Fatty Acid biomarkers detection, the diversity index (Shannon-Wiener) was introduced into analyzing the microbial community diversity based on PLFAs. The quantities of PLFAs were clustered by SPSS to analyze the characteristics of microbial community in the rice rhizosphere. 【Result】The results showed that 38 PLFAs biomarkers were detected out, in which there were 23 PLFAs completely distributed and 15 PLFAs incompletely distributed in the rice rhizosphere. The quantities of PLFAs related to bacteria were higher than either that of fungi or tha