L-半胱氨酸壳聚糖电化学传感器对L-抗坏血酸检测及机理研究.pdf

L-半胱氨酸壳聚糖电化学传感器对L-抗坏血酸的检测及机理研究 a *a,b a* a b 谭福能 庞雪辉 隋卫平 张洁 侯保荣 (a 济南大学化学化工学院 济南 250022) b ( 中国科学院海洋研究所 青岛 266071) 摘要 本文通过将壳聚糖和L-半胱氨酸修饰到玻碳电极基底表面，制备了一种新型的电化学传感器，并将此传感器用于对 L-抗坏血酸的测定。通过循环伏安法和交流阻抗法对传感器的电化学特性作了表征，并将交流阻抗实验得到的数据通过等效 电路进行了拟合。还采用计时电流法对L-抗坏血酸在传感器上催化氧化反应的扩散系数和催化速率常数进行了研究。实验结 果表明：此电化学传感在pH5.0 的乙酸-乙酸钠缓冲溶液中对L-抗坏血酸具有良好的电化学响应，通过线性伏安实验发现： -5 -3 1 AA 的氧化峰电流与其浓度在 1.0×10 ～2.0×10 mol/L 的范围内成良好线性关系范 围内呈现良好的线性关系，检出限为 4.3×10 -6mol/L ，且该传感器具有良好的重现性和稳定性，并将其成功运用于对维生素C药片中L-抗坏血酸含量的检测。 关键词 L-半胱氨酸；壳聚糖；电化学传感器；L-抗坏血酸 L-cysteine/chitosan electrochemical sensor for determination of L-ascorbic acid Tan, Funenga Pang, Xuehuia,b Sui, Weipinga* Zhang, Jiea Hou Baorongb a ( College of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022) b ( Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071) Abstract This paper described the determination of L-ascorbic acid (AA) using a new kind of electrochemical sensor by modified glassy carbon electrode(GCE) with a mixture of L-cysteine and chitosan. This electrochemical sensor was analyzed by electrochemical impedance spectroscopy(EIS) and Cyclic voltammetry(CV) were used to investigate the electrochemical performances. The measured EIS data were fitted with an equivalent circuit. Kinetic parameters of AA on sensor such as diffusion coefficient and catalytic rate constant were determined by Chronoamperometry(CA). The results show that electrochemical sensor showed excel