猪整合素beta;1基因CRS-PCR多态性与产仔数的关联性分析.pdfVIP

HEREDITAS (Beijing) 2012 7 , 34(7): 879―886 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2012.00879 猪整合素β基因CRS-PCR多态性与产仔数的关联性分 1 析 郭良勇, 傅金銮, 王爱国 , , , 100193 文章采用CRS-RFLP技术对长白猪、大白猪和杜洛克猪 3 个品种的整合素β 基因第 5 外显子T32207C位 1 点及第7 外显子A35230G位点进行单核苷酸多态性分析, 并将基因多态性与猪的产仔数进行关联分析。结果表 明：32207 多态位点的基因型效应对3 个品种的总产仔数(TNB)和产活仔数(NBA)影响均不显著; 35230 多态位 点的基因型效应对大白猪和长白猪头胎、二胎及所有胎次的TNB和NBA 的影响达到显著(P0.05)或者极显著水 平(P0.01), 基因型GG、AG 与AA 对产仔数的影响存在差异, 其效应为GG, AG AA 。可见整合素β1基因35230 位 点的G等位基因对大白猪和长白猪的产仔数性状有显著影响。 CRS-PCR; 整合素β 基因; 多态性; 产仔数; 猪 1 Analysis of association between CRS-PCR polymorphisms of in- tegrin β gene and litter size in pigs 1 GUO Liang-Yong, , FU Jin-Luan, WANG Ai-Guo College of Animal Science and Technology, China Agricultural University, National Engineering Laboratory for Animal Breeding, The Key Laboratory of Animal Genetics and Breeding of Ministry of Agriculture, Beijing 100193, China Abstract: Single nucleotide polymorphisms of exon 5 T32207C and exon 7 A35230G of integrin β gene were detected 1 in Landrace, Large White and Duroc by CRS-RFLP. Association between the polymorphism and litter size was analyzed by the method of least square means. At 32207 polymorphic loci, there was no significant difference on TNB and NBA be- tween the genotypes in Landrace, Large White, and Duroc. At 35230 polymorphic loci, there was significant difference (P0.05) or greatly significant