中国荷斯坦牛CVM的基因检测及其与产奶性状的关联分析.pdfVIP

HEREDITAS (Beijing) 2010 7 , 32(7): 732―736 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2010.00732 中国荷斯坦牛CVM 的基因检测及其与产奶性状的关联 分析 1, 2 1 1 1 1 1 初芹 , 张毅 , 孙东晓 , 俞英 , 王雅春 , 张沅 1. , 100193; 2. , 100097 脊椎畸形综合征(Complex vertebral malformation, CVM)是由位于牛第3 号染色体(BTA3) 的SLC35A3 基因 外显子 4 的一个单碱基突变(G559T)所致。该致病基因在世界许多国家的荷斯坦牛群中都有一定的比例。文章 对北京地区38 头优秀种公牛进行分析, 发现了4 头携带者, 进而检测了这些携带者公牛的555 头女儿的基因型, 其中携带者占检测母牛数的 44.0% 。此外, 关联分析结果表明, 携带者母牛与非携带者母牛的生产性能之间存 在显著差异(P0.01) 。携带者母牛的 5 个产奶性状育种值均显著高于非携带者, 泌乳持续力和体细胞评分 SCS 的育种值也比非携带者略高。CVM 致病基因可能与BTA3 上影响产奶性状的QTL 或基因连锁。因此, 建议生 产中对CVM 携带者进行逐步淘汰。 脊椎畸形综合征; SLC35A3 基因; 中国荷斯坦牛; 引入酶切位点聚合酶链式反应(PIRA-PCR); 关联分析 Identification of the complex vertebral malformation gene in Chinese Holstein and its association with dairy performance traits 1, 2 1 1 1 1 1 CHU Qin , ZHANG Yi , SUN Dong-Xiao , YU Ying , WANG Ya-Chun , ZHANG Yuan 1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China; 2. Institute of Animal Husbandry and Veterinary Medicine , Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China Abstract: Complex vertebral malformation is caused by a single base mutation from G to T at the nucleotide position 559 in the bovine solute carrier family 35 member 3 (SLC35A3) gene exon 4 on Bos Taurus autosome (BTA) 3. The presence of the disease gene in Holstein dairy cattle has been reported in many countries. In this study, we examined 38 top Chinese Holstein sires in Beijing, four of which were identified as CVM carriers. Furthermore, 555 daughters of the four CV sires were examined and 44.0%