成肌细胞在基因治疗和组织工程中应用研究综述.pdfVIP

中国修复/990314 中国修复重建外科杂志 CHINESE JOURNAL OF REPARATIVE AND RECONSTRUCTIVE SURGERY 1999年 第13卷　第3期 Vol.13　No.3 1999 成肌细胞在基因治疗和组织工程中的应用综述 岑石强　杨志明 　　【摘　要】　　目的　探讨骨骼肌成肌细胞的生物学特性及其在基因治疗、组 织 工程方面的应用前景。方法　回顾成肌细胞的有关研究成果，广 泛查阅近期有关成肌 细胞在细胞移植及其与材料复合方面的文献，综述有关内容。结果　目前成肌细胞体 外培养已是一个成熟的方法，其体内与体外的生物学 特性已研究得较清楚。由于它能 融合到宿主的肌纤维内，将目的基因介导到 宿主体内，能稳定表达对应的基因产物， 因此在基因治疗方面有良好的应用前景。现已在 遗传性肌营养不良症及一些遗传性血 液病方面有广泛研究。它与胶原等生物材料有良好 的生物相容性，体外能在胶原的诱 导下良好地分化，也有将其用于血管、心肌及骨骼肌组织 工 程方面的研究。结论　成 肌细胞以其特有的生物学特性将会广 泛用于 基因治疗及组织工程中。 　　【关键词】　　成肌细胞　　细胞移植　　基因治疗　　 组织工程 A REVIEW ON THE APPLICATION OF MYOBLAST ON GENE THERAPY AND TISS UE ENGINEERING CEN Shi-qiang, YANG Zhi-ming. Departmen t of Orthopedic Surgery, the First University Hospital, West China University of Medical Sciences. Chengdu Sichuan, P.R. China 610041. E-mail: orthop ＠ 　　【Abstract 】　　Objective　 Because of its special biological characteristics, myoblast might play a role in gene delivery and cell-to-biomaterial interactions. In th is paper, the biological features of myoblast and its application on gene therap y and tissue engineering was discussed.Methods　Docum ents about proliferation and differentiation of myoblast were reviewed in detail s. The prospects of its application on gene therapy and tissue engineering were also presented. Results　 Myoblast wa s important in muscle regeneration. The activation of myoblast to proliferate an d differentiate was the very beginning of regeneration after injury. The cul tured myoblast had high potential to proliferate, it was ready to fuse with each other and to form myotube (the spec ial behavior of myoblast differentiation). Myoblast transplantation had been st udied as a possible treatment for