2型糖尿病外周血内皮祖细胞的诱导分化及影响因素的研究.pdfVIP

188 2006 14 3 Chin J Diabetes, June 2006, Vol 14, No 3 2 王爱民 雷闽湘 刘泽灏 汤叁娥 唐国华 2 ( T2DM ) ( endothelial progenitor cell, EPC) 20 T2DM 19 T2DM 21 EPC ,, EPC EPC (P 005) ( P 001) EPC SBP FP ( R = - 0266, P 005; R = - 0619, P 001) , EPC HbA c ( R = - 0749, P 1 005) ,(R = - 0406, P 001) FP SBP EPC ,T2DM EPC , HbA cSBP 1 ,2 ;; The induction and differentiation of endothelial progenitor cell from human peripheral blood of type 2 di- abetics and its influential factors WA N G A i- min , L EI M in-x iang , L I U Ze- hao , et al Dep ar tment o E ndocr inology , X iangy a H osp ital , Center South Univ ers ity , Changs ha 4 10008 , Ch ina Cor r esp onding author : L EI M in-x iang Abstract Objective T o isolate and identify human endothelial progenitor cell(EPC) from pe- ripheral blood of type 2 diabetic patients and investigate the capability of differentiation and prolifera- tion of EPC Methods Mononuclear cells isolated from human peripheral blood of type 2 diabetic patients ( diabetics without complication,n = 20;diabetics with angiopathy,n = 19) and control subjects ( n = 21) were culturedT he appearing time and the number of cell clusters and spindle-shaped cells were observed respectively Attached spindle-shaped cells were detached and labeled with a series of antibodies against blood vessel endothelia-l specific markers Fluorescence-activated cell sorting ( FACS) detection of EPC was performed on attached spindle cells after 7 days Results The num- ber of EPC and EPC-bearing clusters in diabetic vascular complication group were significantly fewer than those of nondiabetic control subjects and diabetics without complication (P 001) T he number of clusters were inve