呼和浩特地区某奶牛场奶牛乳腺炎病原菌的分离鉴定和药敏试验.pdf

Hohhot Region a Separation of Dairy cow Mastitis Pathogen Identification and Susceptibility Test Abstract Bovine mastitis is one of the most common diseases of dairy cow breeding, cause huge economic losses, which is influenced by many factors, of which the pathogenic microorganisms infection is one of the main. Experimental objective ：This experiment from actually, combined with clinical practice ，collecting Milk sample from a dairy farm.Counting on milk somatic cell count and differential counting, The separation of bacterial culture, identification and drug susceptibility test.Experimental results of the data then fed back to cow, guide the clinical medication and breeding management. Experimental methods: Using somatic cell count meter somatic cell count on milk samples with Newmans dyeing method, somatic cell count under the microscope.Willing milk samples were inoculated on blood AGAR medium, QueFuMan and MaiKangKai culture medium for separation of the bacteria culture.Cultivating the typical biochemical identification of bacteria, staphylococcus aureus five biochemical identification of genus, 14 biochemical identification of E. Coli. Identificated the bacteria respectively made 21 kinds of drug susceptibility test. The experimental results:Collecting the 80 cases of milk samples , somatic cell count in under 2 x 105 are 34 cases, between the 2 x 105 ~ 5 x 105 are 9 cases and more than 5 x 105 are 37 cases. The incidence rate of mastitis is 57.5%, the Subclinic mastitis incidence rate is 11.25% , The Subclinic mastitis take mastitis incidence rate is about 19.57%