利用噬菌体随机肽库筛选抗柯萨奇病毒B3多肽的研究.pdfVIP

第 21 卷 3 期 中 国 病 毒 学 21 （3 ）：281-283 2006 年 5 月 VIROLOGICA SINICA May 2006 利用噬菌体随机肽库筛选抗柯萨奇病毒 B3 多肽的研究 ** 刘 念 １ ，李 凡 １ ，远 航 ２ （1.吉林大学基础医学院病原生物教研室，吉林长春，130021；2.吉林大学第二医院肾病内科，吉林长春，130041） Anti-CVB3 Polypeptides Selected from A Phage Display Random Peptide Library 1 1** 2 LIU Nian ，LI Fan ，YUAN Hang （1. Dept.of Pathogenobiology, School of Basic Medical Sciences, Jilin University, Changchun 130021, China; 2. Dept. of Nephrology, No. 2 Hospital, Jilin University, Changchun 130041，China） Abstract ：CVB3 was propagated in Hep-2 cells and purified by sucrose gradient centrifugation. The purified CVB3 reacted with random peptides library displaying 9 amino acids. After 3 rounds of screening, the capability of phage peptides in the inhibition of CVB3 replication was determined. DNA was extracted from the positive phage clones, sequenced and the amion acid sequence was deduced. The results showed that 3 phage positive peptides were identified and bound to CVB3 with high affinity. The binding of the peptide to CVB3 was shown to inhibit replication substantially. The TCID50 of progeny -7.5 -5.25 -6 -5.5 virus was reduced from 10 SFU/mL to 10 , 10 , 10 SFU/mL by the three peptides, respectively. The data demonstrate that peptides against CVB3 can be selected from phage peptide library and may prove useful as antiviral peptides agents. Keywords ：CVB3 ; Phage peptide libray ; Antivi