猪瘟病毒猪细小病毒猪繁殖与呼吸综合征病毒猪_省略_狂犬病病毒多重PCR方法的建立.pdfVIP

Chinese Journal of Veterinary M edicine 2007 (43 ) 2 3 PCR 赵　耘, 秦玉明, 张广川, 赵启祖, 宁宜宝, 戴志红, 谢　磊 ( , 100081) : , 4 4PCR , , : CSFV 10 T CID50 PPV, 10 T CID50 PRRSV, 1 TCID50 PRV, 100 T CID50 CSFV 10 T CID50 PPV, 10 , 1 , 100 , TCID50 PRRSV T CID50 PRV TCID50 PRV A PPV PRRSV KY35 PRRSV B13 6 ,BVDVBDV 4 : ; ; ; ; PCR 中图分类号: S858. 285. 3　　　文献标识码:A　　　文章编号:0529-6005( 2007) 02-0003-04 Establishment and application of the multiplex PCR to the detection of CSF PP PRRS and AD viruses, , , - , - , - , Z AO Yun QIN Yu ming ZhANG Guang chuan Z AO Qi zu NING Yi-bao, DAI Zhi-hong, XIE Lei (China Institute of Veterinary Drug Control,National Classical Swine Fever Reference Laboratory,Beijing 100081, China) : ( ) , ( ) , Abstract classical swine fever virus CSFV porcine parvoviruses PPV porcine reproductive and res- piratory syndrome virus(PRRSV) and Aujeszkys diseasevirus(ADV) all can result in reproductive failure of swine, which causes significant economical losses to the swine industry on a world-wide scale. Four primers . 10 50, 10 50, were designed and the multiplex PCR were developed Its sensitivity were CSFV TCID PPV T CID PRRSV 1 T CID50,PRV 100 TCID50 CSFV 10 TCID50,PPV 10 T CID50,PRRSV 1 TCID50,PRV 100 T CID50. The multiplex PCR for CSFV Shimen strain, CSFV lapinized virus strain, PRV Minnan A strain, PPV, 35 13 . PRRSV ky strain and B strain were done by this method The DNA fragment were obtained from the six viral strains but not from BVDV and BDV.