聚多钯胺在生物学中的应用2.pdfVIP

2810 Biomacromolecules 2010, 11, 2810–2817 Antifungal Nanoparticles and Surfaces Cristiana S. O. Paulo,†,‡ Maria Vidal,§ and Lino S. Ferreira*,†,‡ Center of Neurosciences and Cell Biology, University of Coimbra, 3004-517 Coimbra, Portugal, Biocant, Biotechnology Innovation Center, 3060-197 Cantanhede, Portugal, and Department of Pure and Environmental Sciences, ESAC, Bencanta, 3040-316 Coimbra, Portugal Received August 2, 2010; Revised Manuscript Received August 31, 2010 Nosocomial fungal infections, an increasing healthcare concern worldwide, are often associated with medical devices. We have developed antifungal nanoparticle conjugates that can act in suspension or attach to a surface, efficiently killing fungi. For that purpose, we immobilized covalently amphotericin B (AmB), a potent antifungal agent approved by the FDA, widely used in clinical practice and effective against a large spectrum of fungi, into silica nanoparticles. These antifungal nanoparticle conjugates are fungicidal against several strains of Candida sp., mainly by contact. In addition, they can be reused up to 5 cycles without losing their activity. Our results show that the antifungal nanoparticle conjugates are more fungistatic and fungicidal than 10 nm colloidal silver. The antifungal activity of the antifungal nanoparticle conjugates is maintained when they are immobilized on a surface using a chemical adhesive formed by polydopamine. The antifungal nanocoatings have no hemolytic or cytotoxic effect against red blood cells and blood mononuclear cells, respectively. Surfaces coated with these antifungal n