儿童口腔尖锐湿疣的临床表现及HPV检测.pdfVIP

　　　　　　　　　　　　　　　　　华西口腔医学杂志　WCJS 　2002 年 2 月第 20 卷第 1 期 ·27 · 儿童口腔尖锐湿疣的临床表现及 HPV 检测 何志秀 　刘来奎 　陈 　艳 摘要 　目的 :探讨儿童口腔粘膜尖锐湿疣的病毒类型、传播途径、临床病理特点及预后等。方法 : 回顾 6 例被确诊 为口腔粘膜尖锐湿疣患儿的临床特点及 HE 切片 ,并对其中 5 例采用免疫组化染色及原位杂交检测人乳头状瘤病 ( ) 毒 HPV DNA 。结果 :儿童口腔尖锐湿疣多发生在 2 岁左右 ,发病部位多位于腭部 ,并且多数有家族感染史 ,镜下见 棘层上部或角化层常出现灶性凹空细胞 , 免疫组化检测结果显示 5 例 HPV 共同抗原全部阳性 ,5 例中有 4 例 HPV1618- E6 阳性;原位杂交结果显示仅 1 例 HPV6 和 HPV11 同时阳性 ,另 1 例初发时 HPV6 阳性而复发后呈阴性。 结论 :儿童口腔尖锐湿疣的病毒类型、传播途径可能与成人不同。 关键词 　 ( ) 口腔尖锐湿疣 　人乳头状瘤病毒 HPV 　儿童 　原位杂交 A Retrospective Study of Clinical Manifestation and Human Papilloma Virus Detection of Oral Condyloma Acuminate in Children He Zhixiu , Liu Laikui , Chen Yan College of Stomatology , West China University of Medical Sciences Abstract Objective : The aim of this study is to study genotype , transmission , clinical and pathological characteristics , and prognosis of oral condyloma acuminate (CA) in children. Methods : The authors retrospected the clinical characteristics and slices of HE staining of six cases which have been diagnosed as oral CA and , performed immunohistochemistry ( IHC) and in situ hybridization ( ISH) analysis to detect the DNA of human papilloma virus in 5 cases. Results : Oral CA often happened in the hard or soft plates of children of two-year-old. Most of them came from the families had been infected by human papilloma virus ( HPV) . Histological examination demonstrated that koilocytes were common in the upper spinous and corneal layers. HPV was detected in all cases. HPV1618- E6 antigen was positive in 4 of 5 cases examined. The result of ISH only s