Selection Under Domestication Evidence for a Sweep in the Rice Waxy Genomic Region文献.pdfVIP

Copyright 2006 by the Genetics Society of America DOI: 10.1534/genetics.106.056473 Selection Under Domestication: Evidence for a Sweep in the Rice Waxy Genomic Region Kenneth M. Olsen,*,1 Ana L. Caicedo,* Nicholas Polato,† Anna McClung,‡ Susan McCouch† and Michael D. Purugganan*,2 *Department of Genetics, North Carolina State University, Raleigh, North Carolina 27695, †Department of Plant Breeding and Genetics, Cornell University, Ithaca, New York 14853-1901 and ‡ USDA–Agriculture Research Service, Beaumont, Texas 77713 Manuscript received January 28, 2006 Accepted for publication March 15, 2006 ABSTRACT Rice ( Oryza sativa) was cultivated by Asian Neolithic farmers .11,000 years ago, and different cultures have selected for divergent starch qualities in the rice grain during and after the domestication process. An intron 1 splice donor site mutation of the Waxy gene is responsible for the absence of amylose in glutinous rice varieties. This mutation appears to have also played an important role in the origin of low amylose, nonglutinous temperate japonica rice varieties, which form a primary component of Northeast Asian cuisines. Waxy DNA sequence analyses indicate that the splice donor mutation is prevalent in temperate japonica rice varieties, but rare or absent in tropical japonica, indica, aus, and aromatic varieties. Sequence analysis across a 500-kb genomic region centered on Waxy reveals patterns consistent with a selective sweep in the temperate japonicas associated with the mutation. The