异黄酮改构化合物F11 雌激素效应初步研究论著.docVIP

异黄酮改构化合物F11雌激素效应初步研究 论著 陈诚1，1，艾国平2，常青400038 重庆第三军医大学西南医院妇产科军事预防医学院全军复合伤研究所创伤、烧伤与复合伤国家重点实验室 [摘要] 目的 初步研究异黄改构化合物F11的雌激素效应。方法 构建去双卵巢大鼠动物模型，经腹腔注射F11[15、50、150 mg/(kg·d )3个剂量组（F11-15组、F11-50组、F11-150组）]共10周，同时设置阴性对照组，阳性对照组（E2）和假手术组（Sham），统计分析各组大鼠子宫湿质量/体质量情况，HE染色观察用药前后阴道涂片和子宫内膜变化情况；Western blot检测ERα和ERβ的表达情况。 结果 F11-50及F11-150P＜0.0511-150组大鼠子宫湿质量/体质量与E2和假手术组无显著差别(P＞0.05F11-150组与E2和假手术组相似。F11各组较阴性对照组子宫内膜腺体数目增多，腺腔增大，F11-150组与E2和假手术组相似。F11各组ERα及ERβ蛋白表达均强于阴性对照组(P＜0.05ERα表达弱于E2和假手术组(P＜0.01)ERβ的表达强于E2和假手术组(P＜0.0)。结论 F11在增加去双卵巢大鼠子宫湿质量、阴道上皮成熟及子宫内膜ER的表达等方面均表现出雌激素ERβ介导。雌激素受体调节剂Preliminary Study of Estrogenic Effect of Isoflavone Derivative F11 in Vivo Chen Cheng, Wang Lin, Ai Guoping, Chang Qing (1Department of Gynaecology and Obstetrics, Southwest Hospital; 2State Key Laboratory of Trauma, Burns and Combined Injury, Institute of Combined Injury, College of Military Preventive Medicine, Third Military Medical University, Chongqing, 400038, China) [Abstract] Objective To study the estrogenic effect of isoflavone derivative F11 in vivo. Methods The ovariectomized rat models were constructed, and the rats were divided into a negative control group (normal saline), a positive control group (estradiol, 50 ?g/kg·d), a sham operation group and three F11 groups including a F11-15 group, a F11-50 group and a F11-150 group (intraperitoneal injection of F11 with 15, 50 and 150 mg/kg·d, respectively). After the treatment for 10 weeks, the ratio of rat uterus wet weight/body weight was analyzed, and the vaginal smear and endometrium were observed by HE staining. The expression of estrogen receptor (ER) α and ERβ in endometrial tissues was determined by Western blot. Results The ratio of rat uterus wet weight/body weight in the F11-50 group and F11-150 group was significant higher than that of the negative control group (P0.05), but the ratio of the F11-150 group was not significantly different from that of the positive control group or sham operation group (P0.05).