Generation and Phenotype Analyses of TRPV2 Conditional Knockout Mice.docVIP

TRPV2条件性基因剔除小鼠的建立及其基本表型分析 王瑶莉1 张梦杰1* olfgang Schwarz3 蔡 蕾2 匡 颖2 费俭12 (1同济大学生命科学与技术学院上海 2000922上海南方模式生物研究中心上海 2012033上海针灸经络研究中心上海 201203 摘要 TRPV2 是瞬时感受器离子通道蛋白亚家族成员之一, 主要分布在感受伤害性热痛刺激和机械刺激的有髓Aδ神经和少部分C神经纤维中, 在机体的热痛感觉、机械刺激感觉过程中发挥着重要的作用。此外, 该基因在脑和多种非神经组织中也有表达, 参与维持体内Ca2+、Mg2+的离子平衡, 暗示了其功能的多样性[1]。研究建立了TRPV2基因条件性剔除小鼠模型, 并研究了TRPV2基因全身性剔除小鼠在体重、血压、学习记忆、运动协调能力等方面的表型变化, 结果显示, 与野生型相比, 纯合突变小鼠的出生率明显偏低, 但在上述指标方面并无显著差异。 关键词 TRPV2; 条件性基因敲除; 发育; 体重; 行为 and Phenotype Analyses of TRPV2 Conditional Knockout Mice Wang Yaoli1, Zhang Mengjie1*, Wolfgang Schwarz3, Cai Lei2, Kuang Ying2, Fei Jian1,2 (1School of Life Science and Technology, Tongji University, Shanghai 200092, China; 2Shanghai Research Center for Model Organisms, Shanghai 201203, China; 3Shanghai Research Center for Acupuncture Meridian, Shanghai 201203, China) Abstract TRPV2, one member of the TRP cationic channels family, mostly distributes in myelinated Aδ neurons and C fiber neurons. It was considered to play an important role in the mechanical and noxious heat sensation. TRPV2 was also detected in many non-neuronal tissues which maintained the ionic equilibrium of principal Ca2+ and Mg2+and indicated its various functions in vivo. For further TRPV2 functional studies, TRPV2 conditional knockout mouse strain was constructed by using Cre-loxP method in our lab. Several basic phenotype analyses were conducted in the TRPV2 knockout mice. The results showed there were no differences in the bodyweight, blood pressure, study, memory and motor coordination between TRPV2 knockout mice and WT mice while the birth rate was lower in KO mice. Key words TRPV2; conditional gene knock out; phenotype analysis; development; body weight; behavior 收稿日期: 2012-11-12 接受日期: 2013-01-07 *通讯作者 Received: November 12, 2012 Accepted: January 7, 2013 *Corresponding author. Tel: +86-21 E-mail: cherry5652@tongji.edu.cn 感受外界环境变化的能力对于生物体是相当重要的。哺乳动物中, 外界刺激的传入依赖于表皮上的一些特异的蛋白, 其中位于细胞膜上的非选择性阳离子通道蛋白, 也称为瞬时感受器离子通道蛋白家族transient recepto