高锰胁迫与低pH 对黄瓜根系氧化胁迫和抗氧化酶的复合效应.pdfVIP

中国农业科学 2005,38(5):999-1004 Scientia Agricultura Sinica 高锰胁迫与低pH对黄瓜根系氧化胁迫和抗氧化酶的复合效应 史庆华, 朱祝军, 李 娟, 钱琼秋 （浙江大学园艺系/农业部园艺植物生长发育与生物技术重点开放实验室，杭州 310029 ） 摘要：以黄瓜为材料，研究了pH 6.5和pH 4.5条件下高锰胁迫对根系氧化胁迫和抗氧化酶活性的影响。结 果表明，随着锰浓度的提高，黄瓜根系膜脂过氧化产物MDA的含量呈明显的上升趋势，降低pH进一步加剧了膜脂 过氧化程度，导致黄瓜的根系和地上部生长均受到明显抑制。高锰胁迫下Mn-SOD和Fe-SOD活性增加，在清除O - 2·方 面发挥重要作用。CAT对高锰胁迫比较敏感，高锰和低pH 处理导致其活性明显下降，而GPX以及抗坏血酸－谷胱 甘肽循环过程中的重要酶类 APX、DHAR 和 GR 在低 pH 和一定范围的高锰处理下活性上升，对降低高锰胁迫下活性 氧伤害具有重要作用。 关键词：黄瓜；锰；胁迫；pH；抗氧化酶 Combined Effects of High Level of Mn and Low pH on Oxidative Stress and Antioxidant Enzymes in Cucumber Roots SHI Qing-hua, ZHU Zhu-jun, LI Juan, QIAN Qiong-qiu (Department of Horticulture, Zhejiang University/Key Laboratory of Horticultural Plant Development and Biotechnology, Ministry of Agriculture, Hangzhou 310029) Abstract: Effects of high level of Mn on oxidative stress and antioxidant enzymes in cucumber roots under different pH (pH 4.5 and pH 6.5) were studied. The results indicated that lipid peroxidation increased seriously with the increasing concentration of Mn, especially under low pH. As a results, the growth of cucumber roots and shoots was significantly inhibited. High level of Mn led to an elevation in activities of Mn-SOD and Fe-SOD, which played an important role in removing O − 2 · CAT was sensitive to high level of Mn, and its activity significantly decreased under high level of Mn and lower pH，while the activities of GPX, APX, DHAR and GR increased under low pH and some extents of high level of Mn, which indicate their important role in scavenging reactive oxygen in cucumber roots tolerance to low pH and high le