消疹止痒胶囊中有效成分的定性鉴别及含量测定.docVIP

消疹止痒胶囊中有效成分的定性鉴别及含量测定 红梅1，王栋2，张勇1*，包丽丽1（010010 呼和浩特，内蒙古自治区人民医院药剂科1；内蒙古食品药品检验所中药科2） 【摘要】 目的：对消疹止痒胶囊中两种有效成分进行定性鉴别并测定阿魏酸的含量。方法：采用薄层色谱法（thin layer chromatography,TLC）对方中的诃子、麻黄进行定性鉴别，采用高效液相色谱法（HPLC）测定川芎中阿魏酸的含量。结果：该鉴别方法专属性强，薄层色谱斑点清晰，阴性对照无干扰；阿魏酸的HPLC色谱峰与其他色谱峰分离良好，进样量在0.0538μg～ 0.3228μg范围内呈良好线性关系（r=0.9999），平均加样回收率为100.30%，RSD=0.96%（n=9）。结论：本方法简便、准确、重现性好，可作为消疹止痒胶囊的质量控制方法。 【关键词】消疹止痒胶囊；阿魏酸；薄层色谱法；高效液相色谱法；质量标准 Identification of active ingredients and content determination in xiaozhenzhiyang Hong Mei1, Wang Dong2, Zhang Yong1, Bao Lili1 (1Department of pharmacy, Mongola Hospital, 2Departmant of Chinese Medicine, Mongola for Food and Drug control, Huhhot, 010010, China) [Abstract]Objective: To identify two active ingredients and determine the content of ferulic acid in xiaozhenzhiyang capsules. Methods: Thin layer chromatography (TLC) was used for qualitative identification of chebula Fructus, Ephedrae Herbap; The content of Ferulic acid from chuanxiong Rhizoma was determined by HPLC. Results: The identification method was highly specific with clear TLC spots. The negative control did not impose any disturbance. In HPLC, the chromatographic peah of ferulic acid was well isolated from othe peahs. There was a good linear relationship between the sample size and the peak area of piperine in a range of 0.0538μg - 0.3228μg(r=0.9999). The average recovery of ferulic acid was 100.30%(n=9), RSD=0.96%. Conclusion: The method is simple accurade and good reproducibility. lt can be used for the quality control of [基金项目] 内蒙古科技厅计划项目 （ *[通信作者] 张勇，电话：（0471）6620289； Email:zyong69@126.com xiaozhenzhiyang capsules. [Key words]xiaozhezhiyang capsules; Ferulic acid; thin layer chromatography; high performance liquid chromatography; quality standard 急慢性荨麻疹是皮肤出现风团，时隐时现，伴有瘙痒反复发作的一种皮肤病，属于中医风疹块、隐疹等范畴，为过敏性皮肤疾患。中医学认为皮肤瘙痒是因肌表卫气虚弱，劳汗当风，或风寒，或风热，或风湿之邪入侵肌肤，不得疏泄所致，亦可风、燥、湿、热久留体内化火生燥，以致津血枯涩，肌肤失养而致，故采用凉血润燥，调和营卫，清热燥湿法则治疗。随着社会现代化进程的加速，微生物、物理、化