新近红外染料5,5′-二羧基-1,1′-二磺丁基-3,3,3,′3′-四甲基吲哚三碳菁分光光度法直接测定.pdfVIP

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2017-09-01 发布于安徽
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新近红外染料5,5′-二羧基-1,1′-二磺丁基-3,3,3,′3′-四甲基吲哚三碳菁分光光度法直接测定.pdf

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Spectrophotometric Determination of Albumin in Serum Using a Novel Near-Infrared Cyanine Dye, 5,5’-Dicarboxy-1,1’-Disulfobutyl-3,3,3’,3’- Tetramethylindotricarbocyanine Wei-Rong Li, Xiao-Feng Guo, Hua-Shan Zhang, Hong Wang*** Department of Chemistry, Wuhan University, Wuhan 430072, P.R.C E-mail：wanghong@ Abstract Near-infrared (NIR) dyes (λ 650 nm) are very promising for the analysis of biological em samples, because their long wavelengths allow for minimization of interferences from biomolecule matrices. In this paper, a novel NIR dye, 5,5’-dicarboxy-1,1’-disulfobutyl-3,3,3’,3’-tetramethylindotricarbocyanine (DCDSTCY) has been developed for the spectrophotometric assay of total proteins in serum. Under acidic conditions, the binding of DCDSTCY to proteins caused a new peak at 878 nm, the height of which was proportionable to the concentration of proteins. The linear range was found to be 0.04-0.5 µg -1 mL for bovine serum albumin (BSA) and human serum albumin (HAS) with the detection limits of 5 ng mL-1, respectively. The maximum binding number of BSA with DCDSTCY was measured as 133. The proposed method has been applied to the quantitation of total proteins in serum with recoveries of 96.6-104%. Keywords: Near infrared (NIR) dye; 5,5’-dicarboxy-1,1’-disulfobutyl-3,3,3’,3’- tetramethylindotricarbocyanine (DCDSTCY); Spectrophotometry; bovine serum albumin (BSA); human serum albumin (HAS); Se