水稻植株及其内生微生物脂肪酸生物标记异质性_11698.docVIP

水稻植株及其内生微生物脂肪酸生物标记 胡桂萍，刘波*，郑雪芳，(福建省农业科学院农业生物资源研究所，福州市，350003) 摘要 以兴优制5、兴优HK02、准优HK05、准优航653和水稻组培苗为材料，采用改良脂肪酸法分析水稻植物自身脂肪酸和其内生微生物脂肪酸结构组成，结果检测到得到水稻和其内生微生物共有脂肪酸有直链脂肪酸20:0、12:0、16:0、18:0和脂肪酸如18:3ω6с(6,9,12)、19:0 CYCLOω8с、15:1ω6с。水稻内生微生物脂肪酸主要支链脂肪酸，如甲基脂肪酸，羟基脂肪酸等，共有22种如i20:0、20:1 ω9с、12:0 3OH等含量差异很大，。对内生微生物脂肪酸进行得到，遗传距离近的水稻品种其距离也近的为多元分布的为单一分布关键词 水稻 组培苗 内生微生物 脂肪酸eterogeneity of fatty acids contained in the plants from rice and their endophytes Gui-Ping Hu, Bo Liu*, Xue-Fang Zheng, Jianfu Zhang，Hua’an Xie* （Agricultural Biological Resource Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003） Abstract: Distribution characteristics of fatty acids contained in the plants from rice and their endophytes were analysised by modified method of fatty acids with four hybrid rice and regenerated plantlet combinations as materials. The four hybrid rice were xingyouzhi5、xingyouHK02、zhunyouHK05、zhunyouhang653. The results revealed that fatty acids obtained 12:0、16:0、18:0 and 20:0 were detected in plant and endophytes in common. And fatty acids of rice obtained Straight Chain Fatty Acid such as 20:0、12:0、16:0、18:0 and unsaturated fatty acids such as 18:3ω6с(6,9,12)、19:0 CYCLOω8с、a17:0、i16:1 H、16:0 N ALCOHOL、15:1ω6с、i15:0 3OH. 22 kinds of the fatty acids were detected in the endophyes of rice includeiso- and anteiso-methyl-branched fatty acids，hydroxy fatty acids and so on , for example i20:0、20:1 ω9с、12:0 3OH. The contents of the fatty acids from endophytes in different rice were different and they represented completely distrituion and singularity distrituion. The Correspondence ananlysis of the fatty acids from endophytes showed that corresponding distances of the fatty acids were relation with the distances of heredity in rice. Loading chart of correspondence ananlysis displayed that fatty acids were multi- distribution when their corresponding distances were longer than , contrary, they were singularity distribution. Key words：Rice; Regenerated Plantlet; Endophyt