结肠癌组织及转移淋巴结中磷酸化mTOR 与表达及其预后作用.docVIP

结肠癌组织及转移淋巴结中磷酸化mTOR的表达及其预后作用 郭梅梅 广东医学院附属南山医院消化内科[摘要] 目的 探讨p-mTOR在结肠癌组织及转移淋巴结中的表达及其对预后的影响。方法 收集136例结肠癌根治术病例，据淋巴结转移情况分为三组，应用免疫组织化学方法检测各组结肠癌组织及转移淋巴结组织中p-mTOR的表达，结合临床病理资料进行统计分析。结果 免疫组化结果显示p-mTOR广泛表达于结肠癌组织及转移淋巴结中。结肠癌组织表达阳性率为47.1%，其中LN3组的表达阳性率（64.3%）显著高于LN1和LN2组（37.8%和40.8%），结肠癌组织p-mTOR的表达率与淋巴转移程度呈线性相关（P=0.026）。转移淋巴结中p-mTOR的表达阳性率为67.0%，其中LN3组阳性率（85.7%）显著高于LN2组（51.0%，P=0.001）。结肠癌组织与转移淋巴结中p-mTOR表达阳性率呈显著相关（P=0.014）。生存分析显示结肠癌组织p-mTOR阳性表达对于各组病例生存率影响不大。而转移淋巴结p-mTOR阳性表达病例5年生存率均显著低于阴性组（P=0.042/P=0.027），转移淋巴结p-mTOR阳性表达患者预后较差。结论mTOR信号通路在结肠癌发生发展中发挥了重要的作用，结肠癌组织p-mTOR的表达与淋巴结转移程度密切相关，转移淋巴结p-mTOR表达可作为中晚期结肠癌的预后标志，mTOR是结肠癌淋巴转移的重要治疗靶点之一。 [关键词] 结肠肿瘤；TOR丝氨酸-苏氨酸蛋白激酶；基因表达；肿瘤转移；淋巴转移；预后 Prognostic role of phosphorylated mTOR expression in cancer tissues and metastatic lymph nodes in colon adenocarcinoma GUO Mei-mei1 , LIU Shao-xiong2, WANG Yong3,YANG Rong-ping, 1 LIAO Xu1 1 Department of Gastroenterology, Nanshan Affiliated Hospital of Guangdong Medical College, Shenzhen, 518000, China; 2 Pathology department , Nanshan Affiliated Hospital of Guangdong Medical ; 3 Surgery Department of Gastroenterology , Nanshan Affiliated Hospital of Guangdong Medical College [Abstract] Objective To explore prognostic role of phosphorylated mTOR expression in cancer tissues and metastatic lymph nodes in colon adenocarcinoma. Methods A total of 136 patients with colon adenocarcinoma were enrolled and divided into 3 groups according to metastatic lymph node status. Phosphorylated mTOR expression was determined in adenocarcinoma tissues and in metastatic lymph nodes using immunohistochemically staining, and analyzed its association with clinicopathological characteristics. Results p-mTOR was frequently expressed in colon adenocarcinoma tissues and metastatic lymph nodes. p-mTOR expression was observed in 47.1% of the colon adenocarcinoma tissues, in which, the expression rate in LN3 group (64.3%) was significantly higher than that in LN1 and LN2 group (37.8% and 40.8%). A significant correlation was found between