肺再灌注损伤Lung Reperfusion Injury After Transplantation.pptVIP

Gene Therapy Transfection of the donor lung through transtracheal route using a 2nd-generation adenoviral vector Transfection of the gene coding for anti-inflammatory cytokine, human IL-10, reduced ischemia-reperfusion injury, improves lung function in a rat single lung transplant model Human lung protection by gene therapy may soon be possible Take Home Message Donor lung assessment Effect of cold ischemic storage Oxidative stress Sodium pump inactivation Intracellular calcium overload Iron release Cell death Consequences of ischemia reperfusion Up-regulation of molecules on cell surface membrane Release of pro-inflammatory mediators Leukocyte activation Strategies to prevent lung dysfunction Method of lung preservation and reperfusion Clinical evidence in prevention and treatment of lung reperfusion injury Future Strategies One of the major challenges will be to improve the number of donor lungs available for transplantation Thanks for Your Attention Lung Reperfusion Injury After Transplantation References Ischemia-Reperfusion-induced Lung Injury Am J Respir Crit Care Med,2003 Reduced Neutrophil Infiltration Protects Against Lung Reperfusion Injury After Transplantation Ann Thorac Surg 1999 Contents Introduction Donor lung assessment Effect of cold ischemic storage Oxidative stress Sodium pump inactivation Intracellular calcium overload Iron release Cell death Consequences of ischemia reperfusion Upregulation of molecules on cell surface membrane Release of proinflammatory mediators Leukocyte activation Strategies to prevent lung dysfunction Method of lung preservation and reperfusion Clinical evidence in prevention and treatment of lung reperfusion injury Future Strategies Take home message Introduction Non-specific alveolar damage, lung edema, and hypoxemia occurr