安徽省部分地区牛衣原体血清学调查.docVIP

安徽省部分地区牛衣原体的血清学调查 宗贺1潘玲1*刘亚1宁康健2 王力生1韩春阳1（1.安徽农业大学动物科技学院合肥230036；2.安徽技术学院凤阳） 摘要牛衣原体病是由鹦鹉热衣原体引起的一种接触性人兽共患传染病。文章采用间接血凝法（IHA）对安徽省部分地区的535份牛血清进行了衣原体抗体检测，结果：阳性率22.43%（120/535）；4个乳牛场群阳性率75%，个体阳性率23.21%（68/293）；9个肉牛场，群阳性率88.9%，个体阳性率21.49%（52/242）。文章并对不同用途牛、不同年龄段牛、肉牛不同购入来源、肉牛不同送检地区衣原体抗体阳性率进行统计分析。结果表明安徽省的牛群中存在不同程度的衣原体感染。IHA法检测衣原体的抗体简便、快速，适合在基层推广。 关键词牛衣原体病；IHA；抗体检测Investigation on Serology of Bovine Chlamydia in some areas of Anhui Province Zong He1,Pan Ling1*,Liu Ya1,Ning Kangjian2,Wang Lisheng1,Han Chunyang1,Zhang Xiaorong1 College of Animal Science and Technology,Anhui Agriculture University,Hefei,230036；2. AnHui Science and Techonoly University，Fengyang 233100) Abstract： Bovine chlamydiosis is one of the zoonotic diseases caused by chlamydophila psittaci. The antibodies against chlamydia from 535 serum samples of bovine in areas of Anhui Province were examined using an indirect hemagglutination assay (IHA) test. The results showed that the positive rate was 22.43%, the group positive rate was 75% and the individual positive rate was 23.21% in four dairy farms, the group positive rate was 88.9% and the individual positive rate was 21.49% in nine beef cattle farms. The results showed that the infection of chlamydiosis is in different levels in Anhui Province according to the different uses, ages, buying sources and areas detected. All these results confirmed that IHA as an easy and quick serological method could be used for monitoring chlamydia antibody and promoting at grass-root. Keywords: bovine chlamydiosis; indirect hemagglutination assay; antibody monitoring 引言鹦鹉热衣原体主要引起动物的流产、肺炎、腹泻以及人类的肺炎、结膜炎、尿道炎等。国际兽疫局（OIE）将此病列为必须通报疾病，我国列为二类传染病[1]。鹦鹉热衣原体在世界各个地方的分布十分广泛，国内已有[2]。。感染主要临床表现妊娠母牛流产、产弱牛犊或死胎；犊牛肺炎、肠炎、支气管肺炎、结膜角膜炎和关节炎等再者，鹦鹉热衣原体也可以引起人的间质性肺炎和支气管炎等疾病[]。 近年来，国相关报道逐渐增多，但安徽省这方面报道。为了解安徽省内牛衣原体病感染现状，必须开展对牛衣原体的抗体检测，减少因该病导致的经济损失防控措施提供。我们于2011年3月至9月对来自安徽省主要养牛地区的13群共535份牛血清采用间接血凝法（IHA）进行了衣原体抗体的检测。现将检测情况报告如下：1.材料与方法 血清样品 对安徽省主要养牛地区进行随机选点并采血。选择了乳牛场4个，血样293份；肉牛场9个，血样242份；共分离牛血清535份。常规