磷脂酶A1用于大豆油酶法脱胶技术的研究.pdfVIP

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磷脂酶A1用于大豆油酶法脱胶技术的研究.pdf

维普资讯 食品,业斜妓 三 艺 搜 求 Vo1.29..No.05,2008 磷脂酶A用于大 油 法 掇术 究 潘明酷’,柴玉华’,杜 鹏 , (1.东北农业大学成栋学院,黑龙江哈尔滨 150030;2.东北农业大学食品学院,黑龙江哈尔滨 150030) 摘 要:植物油的酶法脱胶是一种新的大豆油脱胶方法。利用新型微生物磷脂酶进行大豆油脱胶,通过对反应时间、 酶浓度、反应温度、pH、含水量、ca“浓度、搅拌速度等单因素实验,研究了操作参数对大豆油脱胶效果的影响,确定了 磷脂酶脱胶的最佳条件:反应时问150min,加酶量0.04%,温度49—52oC.pH4.9—5、2,含水量2.0%一2.5%.添加0.1otol/1 Ca“溶液 1501zL,搅拌速度 125r/min,可使大豆油合磷量降到 lOmg/kg以下。结果表明,LecitaseUltra应用于植物油脱 胶效果好且稳定 ,是一种适宜于X--业化应用的酶种。 关键词:磷脂酶A,,大豆油,脱胶 StudyOnenzymecatalyzeddegummingof soybeanO.IbyaphOSphOIipaSeA1 PAN M ing-zhe ,CHAIYu-hua ,DU Peng ’ (1.ChengdongCollegeofNortheastAgriculturalUniversity,Harbin150030,China; 2.FoodCollegeofNortheastAgrieulturalUniversity,Harbin150030,China) Abstract:Enzymatic degumming is anew techniqueofvegetable oilsdegumming. Enzyme—degumming of soybeanoilwith phOsphOlipase A1,and thefeasibitityofenzyme catalyzed reaction were studied.Bysingle- factortestin whichthefactorswere reactiontime,concentration ofenzyme,reactiontemperature,pH,water content,concentrationofCa andmixingspeed,effectofoperationparametersondegummingperformancewas discussed.The optimum process conditions ofenzyme—catalyzed degumming ofsoybean oilwere:enzyme dosage4% ,thetemperature49~52oC,the pH4.9—5.2,watercontent2%~2.5% ,150~L0.1mol/LCa“ solution, mixing speed 125r/min.Theresidualphosphoruscontentdecreasessteadilyto lessthan 10mg/kg. Inlaboratory testsLecitase Ultra has proven to be a kind ofmore suitable phOsphOlipase used to soybean oils enzymatic degumming. Keywords:phOsphOlipaseAI;soybeanoil;degumming 中图分类号:TS225.13 文献标

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