辣椒花药培养再生株群体染色体倍性构成多样性.pdfVIP

华北农学报 ·2 0 0 7 , 2 2 ( 1) : 12 3 12 8 辣椒花药培养再生株群体染色体倍性构成的多样性 1 1 1 2 1 1 陈　斌 ,赵 　泓 ,耿三省 ,张宝玺 ,张月云 ,刘 　凡 ( 1 北京市农林科学院蔬菜研究中心 ,北京 　100097 ;2 中国农业科学院蔬菜花卉研究所 ,北京 　100081) 　　摘要 :采用流式细胞分析术和染色体计数法对辣椒花药培养再生株群体的染色体倍性构成情况进行了鉴定 。显 示了花药培养再生株中染色体倍性构成的多样性 。观察到染色体倍性在不同检测组织器官中的差异现象 ,说明对同 一材料不同器官进行倍性检测以确定植株倍性的必要性 , 以及植株上部器官的染色体倍性对于结籽能力的决定性 。 观察到再生株中个别细胞染色体的丢失现象 。对流式细胞检测技术和染色体计数法的相关性进行了研究 ,得出 2 种 检测技术下二者的吻合度为 095 ,并对流式检测技术中的偏峰现象进行了初步的分析 。 关键词 :辣椒 ;花药培养 ;倍性构成 ;流式细胞仪 ;染色体计数 ( ) 中图分类号 :Q343 　　文献标识码 :A 　　文章编号 :1000 - 7091 2007 01 - 0123 - 06 Studies on Ploidy Composing in Regenerated Plants from Anther Culture of Pepper( Capsicum annuum L . ) 1 1 1 2 1 1 CHEN Bin , ZHAO Hong , GENG Sansheng , ZHANG Baoxi , ZHANG Yueyun ,LIU Fan (1Beij ing Vegetable Research Center ,Beij ing Academy of Agriculture and Foresty Sciences ,Beij ing 　100097 ,China ; 2Institute of Vegetables and Flowers , Chinese Academy of Agricultural Sciences , Beij ing 　100081 ,China) Abstract :Flow cytometric (FCM) analytical technique and chromosome counting were used for the detailed ploidy i dentification in population of anther culture derived pepper plants. Results revealed ploidy diversity in regenerated plants. The different ploidy in organs (eg.Leave and root tip s) of the same plant was observed , and this phenomena showed the importance of determining ploidy level by different samples. Chromosome deletion was observed in some cells of haploid or double haploid plants. The coincidence between FCM and chromosome counting was high up to 0 . 95 in our experiment . We al