大孔吸附树脂富集纯化珠子参总皂苷的工艺研究_王薇.pdfVIP

辽宁中医杂志 2010年第 37卷第 4 期 # 7 13# 1, 2 2 2 1 , , , ( 11 山东中医药大学, 山东 济南 250355; 21 陕西中医学院, 陕西 咸阳 712046) : 目的: 建立大孔吸附树脂富集纯化珠子参总皂苷的最佳工艺条件方法: 以人参皂苷 R 为 照, 以总皂苷 含量为指标, 研究了H PD型大孔树脂吸附和纯化珠子参总皂苷的工艺条件结果: 优化的工艺条件为: 样品溶液的浓度 为 01 13g/mL(生药量/体积); 树脂用量为生药量的 2倍 (质量比) ; 纯化先用水洗至 A - 萘酚反应呈阴性, 改用 6BV 的 60% 乙醇洗脱, 洗脱速度为 2BV /m in结论: H PD- 100型非极性大孔树脂 珠子参总皂苷有良好的吸附及纯化性能; 本 工艺 珠子参总皂苷的纯化收率高且稳定, 纯化后总皂苷含量可达 60% 以上 : H PD型非极性大孔树脂; 珠子参总皂苷; 富集纯化 : R28411 : A : 1000- 1719( 20 10) 04- 0713- 03 Enrichment and Purification Process of Total Saponins from Rhizoma PanacisM ajoris by M acroreticular Resin 1, 2 2 2 1 WAN G W ei , SON G X iao-m e i , KAN G Ya-g uo , LI F eng ( 11Shandong Univ ersity o TCM, J inan 250355, Shandong, Ch ina; 21Shaanx i Univ ersity o TCM , X iany ang 7 12046, Shanx ,i Ch ina) Abstract: O bj ectiv e: T o stablish a proc ssing m thod for nriching and purify ing th total saponins from Rh izom a Panacis M ajoris by M acror ticular R sin.M ethods: W ith gins ng saponin R as th standard samp l and th total sapon ins cont nts as in- d x, to d t rm in th t chnological Conditions for nrich ing and purify ing th total saponins from Rh izom a Panacis M ajoris by HPD M acror ticu larR sin. R esults: Th optmi um t chn ical op rations w r as follows: conc ntration of th samp l w as 0113g /mL ( w ight of th raw m at rial/volum ); volum of th of th r sin w as 2 tmi s w ight of th raw m at rial; purifi d w ith distill d wa- t r until th A- N ap h thol r. show d n gativ , and th n, w ith 60% thanol( 6 tmi s th