应用隶属函数法比较5种菌草抗碱性-2012.10.docVIP

应用隶属函数法比较5种菌草苗期的抗碱性* 林兴生1，林占熺1**，林辉1，林冬梅1，罗海凌1，胡应平1，林春梅1，朱朝枝2 (1. 福建农林大学菌草研究所，福建 福州350002；2. 福建农林大学金山学院，福建 福州350002) 摘要：为探明莱竹（Arundo sp.）、芦竹(Arundo donax )、巨菌草(Pennisetum sp.)、象草(Pennisetum purpureum )、稗草(Echinochloa crusgalli)等5种菌草的抗碱能力，分别以0、50 mmol·L-1、100 mmol·L-1、125 mmol·L-1、150 mmol·L-1、200 mmol·L-1 Na2CO3溶液处理模拟碱胁迫，测定恢复试验后植株死亡率、叶片细胞膜透性、丙二醛（MDA）含量、可溶性糖（SS）含量、超氧化物歧化酶（SOD）活性、过氧化物酶（POD）活性、过氧化氢酶（CAT）活性等生理生化指标，并利用隶属函数法对以上指标进行综合评价，结果表明：稗草、莱竹、象草、巨菌草、芦竹的半致死Na2CO3胁迫浓度分别为107.0 mmol·L-1、127.7 mmol·L-1、 68.5 mmol·L-1、 78.5 mmol·L-1 、123.0 mmol·L-1；随Na2CO3胁迫浓度的升高，5种菌草的相对电导率、MDA呈上升趋势，SS含量、SOD、POD、CAT活性呈先升高后下降的趋势，其生理指标平均隶属函数值分别为0.36、0.95、0.01、0.14、0.83；5种菌草的抗碱能力强弱为：莱竹芦竹稗草巨菌草象草。 关键词：菌草；抗碱性；生理指标 中图分类号：S688.403.7 文献标识码：A 文章编号： Comparison on Alkaline-Resistance of 5 Species of Juncao Seedling by Subordinate Function LIN Xing-sheng1, LIN Zhan-xi1, LIN Hui1, LIN Dong-mei1, LUO Hai-ling1, HU Ying-ping1, LIN Chun-mei1, ZHU Chao-zhi2 (1Juncao Research Institute, Fujian Agriculture and Forestry University, Fuzhou 350002, China; 2Jinshan College, Fujian Agriculture and Forestry University, Fuzhou 350002, China) Abstract: Alkaline resistance of 5 species of Juncao (Echinochloa crusgalli, Arundo sp., Pennisetum purpureum, Pennisetum sp. , Arundo donax) were studied, which were respectively stressed by Na2CO3 solution with different concentration of 0, 50, 100, 125, 150, 200 mmol·L-1 for 5 days. The mortality rate after recovery test was determined. Leaves were analyzed for physiological and biochemical indexes, including soluble sugar content and activity of superoxide dismutase, peroxidase, catalase and memberance permeability, malondialdehyde content. The result indicated that with Na2CO3 concentration increased, soluble sugar content, activity of superoxide dismutase, peroxidase, catalase increased initially then decreased, while relative electrical conductivity and malondialdehyde content increased. The half-lethal Na2CO3 solution concentration of Echino