通心络对实验性家兔主动脉粥样斑块内血管内皮生长因子表达影响.pdfVIP

CN 431262/ R 中国动脉硬化杂志2004 年第 12 卷第 2 期 177 ( ) [文章编号] 2004 1202017706 ·实验研究· 通心络对实验性家兔主动脉粥样斑块内 血管内皮生长因子表达的影响 张 　路 , 　吴宗贵 , 　廖德宁 , 　潘晓明 ( 中国人民解放军第二军医大学附属长征医院心内科 , 上海市 200003) [ 关键词] 　药理学 ; 　通心络对动脉粥样斑块稳定性的影响 ; 　免疫组织化学 ; 　血管内皮生长因子 ; 　氧化型低 密度脂蛋白 ; 　通心络 ; 　动脉粥样硬化 [摘 　要] 　通过构建实验性家兔主动脉粥样硬化模型 ,观察通心络及辛伐他汀对血清氧化型低密度脂蛋白、一氧 化氮、一氧化氮合酶和乳酸脱氢酶及血管形态学的影响 ,并采用免疫组织化学、逆转录 —聚合酶链反应及 Western Blot 蛋白印迹等方法 ,从不同水平观察通心络及辛伐他汀对动脉粥样斑块血管内皮生长因子表达的影响。结果发 现 ,通心络减少动脉粥样斑块血管内皮生长因子表达 ,降低血清氧化型低密度脂蛋白及乳酸脱氢酶浓度 ,提示通心 络可增加粥样斑块稳定性 ,延缓动脉粥样硬化的进程。 [ 中图分类号] 　R96 [文献标识码] 　A Effects of Tongxinluo on the Expression of Vascular Endothelial Growth Factor in Rabbit Aortic Atherosclerotic Plaques ZHANGLu , WU ZongGui , LIAO DeNing , and PAN XiaoMing ( Department of Cardiology , Changzheng Hospital , Second Military Medical University , PLA , Shanghai 200003 , China) [ KEY WORDS] 　　Vascular Endothelial Growth Factor ; 　Oxidized Low Density Lipoprotein ; 　Tongxinluo ; 　Atherosclerosis ; 　Nitric Oxide ; 　Lactate Dehydrogenase [ ABSTRACT] 　　Aim 　To investigate the effects of Tongxinluo and simvastatin on the expression of vascular endothelial growth factor (VEGF) in rabbit aortic atherosclerotic plaques. 　　Methods 　The rabbit aortic atherosclerotic model was developed by high cholesterol feeding. 　The concentrations of oxidized low density lipoprotein (oxLDL) , nitric oxide (NO) , nitric oxide syn thase (NOS) and lactate dehydrogenase (LDH) were determined. 　The levels of VEGF protein in atherosclerotic plaques were studied by means of immunohistochemistry and Western Blot.