谷氨酸受体在噪声所致豚鼠螺旋神经节细胞损伤中的作用.pdfVIP

Acta Physiologica Sinica, February 25, 2007, 59 (1): 103-110 103 * 200433 (1) (NS, 10 µL) NS (10 µL) + (kynurenic acid, KYNA, 5 mmol/L, 10 µL) + 15 NS KYNA 110 dB SPL1 h (auditory brainstem response, ABR)(compound action potential, CAP)N1 (2) NS + ABR CAP KYNA + N1 NS + KYNA + / KYNA R 7 6 4 Role of glutamate receptors in the spiral ganglion neuron damage induced by acoustic noise * ZHANG Yan-Min, MA Bei , GAO Wen-Yuan, WEN Wen, LIU Hai-Ying Department of Physiology, the Second Military Medical University, Shanghai 200433, China Abstract: The aim of the present study was to investigate the role of glutamate receptors in the damage of spiral ganglion neurons (SGNs) induced by acute acoustic noise. This investigation included in vivo and in vitro studies. In vivo , kynurenic acid (KYNA), a broad-spectrum antagonist of glutamate receptors, was applied to the round window of guinea pigs, and its protective effect was observed. The animals were divided into three groups: control (saline, 0.9%, 10 µL), saline (0.9%, 10 µL) + noise and KYNA (5 mmol/L, 10 µL) + noise. Saline and KYNA were applied to the round window membrane with a microsyringe. The animals were exposed to 110 dB SPL of white noise for 1 h. Hearing thresholds for auditory brainstem responses (ABRs) and compound action potentials (CAPs) in all animals were measured before and after treatment. The amplitudes of III waveform of ABR and N1 waveform of CAP and the latency of N1 waveform at different stimulation levels (intensity-amplitude and intensity-latency functions) were also measured. The cochleas were then dissected for transmission electron microscopy (TEM) after final electrophysiological measurement. In vitro , the SGNs of the normal guinea pigs were isolated and