亮菌多糖ATPS-a的抗衰老作用研究.docVIP

亮菌多糖ATPS-1a的抗衰老作用研究 马金宝，沈业寿*，赵根海，卫自 （安徽大学生化微生物研究所，中国 合肥 230039） 摘要：目的：研究亮菌多糖ATPS-1a的抗衰老作用。方法：选用D-半乳糖致衰老模型小鼠，称小鼠体重,测定脾脏、胸腺指数,取血清、肝、脑组织测定SOD、MDA、GSH-Px、NO。结果：亮菌多糖ATPS-1a能明显增加小鼠体重，抑制胸腺指数上升、脾脏指数下降，还能显著提高SOD及、GSH-Px活性并降低MDA、NO含量。结论：亮菌多糖ATPS-1a可通过调节机体免疫功能、清除自由基而发挥其延缓衰老的作用。 关键词：亮菌；多糖；抗衰老；D半乳糖；小鼠 Study on the Effect of Anti-aging of Polysaccharide ATPS-1a from Armillariella tabescens MA Jin-bao,SHEN Ye-shou*，ZHAO Gen-hai Wei i （Institute of Biochemistry and MicrobiologyAnhui University；Anhui Key Laboratory of Research and Development of Chinese Medicine，Hefei 230039，China） ABSTRACT：AIM：To study the anti-aging effects of polysaccharide ATPS-1a from Armillariella tabescens on mouse.METHODS：Regarding D-galactose induced aging mice as research objects，the body weightthymus and spleen index， Superoxide Dismutase (SOD) and Glutathione peroxidase(GSH-Px) activities，Maleic Dialdehyde (MDA) and nitrogen monoxide (NO) levels in serum，liver and brain were measured.RESULTS：Polysaccharide ATPS-1a from Armillariella tabescens could significantly increase the body weight，markedly resist the rising of thymus index and the dropping of spleen index of senile model mice.It could increase SOD and GSH-Px activities obviously，decrease MDA and NO levels dramatically on senile model mice. CONCLUSION：Polysaccharide ATPS-1a from Armillariella tabescens has anti-aging effects by immune-regulatory and eliminating free radicals. KEY WORDS：Armillariella tabescens；polysaccharide；anti-aging；D-galactose；mouse 亮菌（Armillariella tabescens）属真菌界、担子菌门、层菌纲、伞菌目、白蘑科、小蜜环菌 (假蜜环菌)属[1]。它是我国最初发现并拥有自主知识产权的一种真菌。1969年江苏省丹徒县群众发现用当地发光的柳树根水煎剂能治疗胆囊炎，亮菌成为20世纪70年代新发现的药用真菌，子实体可食用，是一种药食兼用的名贵食用菌。中医认为，该菌性寒、味甘、有强筋壮骨、明目、利肺、益肠胃等保健功效，在我国民间流传作为治疗急慢性肝炎及胆道疾患的中草药[2]。目前，国内外对亮菌多糖的研究主要集中在治疗肝炎、防辐射、升白、抑瘤和调节免疫的药理作用上[3-6]，而对亮菌多糖的抗衰老作用还未见报道，本研究拟对亮菌多糖ATPS-1a的抗衰老作用及其机制进行探讨旨在寻求有效的抗衰老剂及自由基清除剂为延缓人体衰老及防治与自由基有关的疾病提供理论依据。 1 材料与方法 1.1 材料 1.1.1 样品与试剂 ATPS-1a(多糖含量为88.76％)由安徽大学糖复合物与糖工程实验室提 供。D-半乳糖，由上海试剂二厂生产。维生素C (VitC)，四川蜀中制药有限公司。SOD、MDA GSH-Px、NO试剂盒均购自南京建成生物