食品中的45种工业染料.pdfVIP

176 2012, Vol. 33, No. 06 食品科学 ※分析检测 - 45 1 2 1 1 2 路 勇 ，渠 岩 ，冯 楠 ，赵俊平 ，金伟伟 (1.北京市食品安全监控中心，北京 100041 ；2.北京市食品酿造研究所，北京 100050) 建立食品中 45 种工业染料残留量的超高效液相色谱 - 串联四极杆质谱快速筛查方法。样品经溶剂提取净化 后，采用 BEH C 18 液相色谱柱分离，以水和乙腈为流动相，进行梯度洗脱，采用多反应监测模式，外标法定量。 结果表明，45 种工业染料的方法定量限为0.1～100μg/kg；线性范围内低、中、高3 个添加水平的回收率为61.51%～ 109.64%，相对标准偏差为5.23%～9.76% 。该方法具有简便快捷、灵敏度高、结果准确等特点，适用于食品中 上述工业染料的快速筛查工作。 工业染料；超高效液相色谱 - 串联质谱；食品；筛查 Rapid Screening of 45 Industrial Dyes in Foods by Ultra Performance Liquid Chromatography-Tandem Quadrupole Mass Spectrometry 1 2 1 1 2 LU Yong ，QU Yan ，FENG Nan ，ZHAO Jun-ping ，JIN Wei-wei (1. Beijing Municipal Center for Food Safety Monitoring, Beijing 100041, China； 2. Beijing Food Brewing Institute, Beijing 100050, China) Abstract：An ultra performance liquid chromatography-tandem quadrupole mass spectrometry (UPLC-MS/MS) method has been developed for the rapid screening of 45 industrial dyes residues in foods. Samples were extracted by solvent extraction and cleaned up before chromatographic separation on a BEH C18 column using a mobile phase consisting of water and acetonitrile by gradient elution. The dyes were analyzed by GC-MS/MS in multiple reaction monitoring (MRM) mode and quantified by the external standard method. The limits of detection for the dyes varied from 0.1 to 100 μg/kg. The average recoveries across high, medium and low spike levels were between 61.51% and 109.64% with a relative standard deviation (RSD) in the range of 5.23% －9.76%. The method was simple, rapid, sens