RP- HPLC 法测定迭鞘石斛中香豆素的含量.pdfVIP

2010 2 8 9 # 50# Journal o f S chuan o f T rad t onal Ch nese M ed c ne V o l12 8, N o1 9, 2010 RP- HPLC * v 周晓梅 夏厚林 黄立华 程 军 张廷模 ( 6 10075) : 目的: 建立 RP-HPLC 法测定迭鞘石斛中香豆素含量的方法方法: 采用 D amons l (钻石) C18 ( 200mm @ - 1 41 6mm, 5Lm ) 色谱柱, 甲醇-水 ( 30B 70) 流动相, 流速 11 0m l# m n , 检测波长 274nm结果: 香豆素线性范 围 011~ 016L g ( r= 019998, n= 6) , 平均回收率 10 1147% ( R SD = 1132% , n= 3) 结论: 该法快速简便结果 可靠精密度好灵敏度高可作 迭鞘石斛中香豆素含量测定的方法及质量控制 : 迭鞘石斛 香豆素 RP - HPLC 含量测定 : R 2841 1 : A : 1000 - 3649 ( 20 10) 09- 0050 - 02 Determ ination of Coumarin in D endr ob inm dennea num by RP- H PLC /Zhou X iaomei X iaH oulin Huang L ihua Cheng Jun Zhang TingmoMChengdu University of TCM (Chengdu Sichuan 6 10075, China) Abstract: O bject ves: To establ sh an RP - HPLC m ethod for the determ nat on o f coum ar n n endrobinm denneanum. M e thods: The sam ples w e re separated on a D amons l C 18 co lum n ( 200mm @ 416mm, 5Lm ) w th the m ob le phase of m etha- no:l w ater ( 30B 70) , the flow ra te of 110m l# m n- 1, and the detect on w aveleng th w as set at 274nm. R esults: T he lnear rang e of 011~ 016L g ( r= 01999 8, n= 6) ; and the average recovery w as 101147% ( RS = 11 32% , n= 3) . Conc lu s ons: T he estab l shed RP - HPLC m ethod w as qu ck, accurate and su tab le for qual ty con tro l of endrobinm denneanum. K ey word s: endrobinm denneanum. conum ar n RP - HPLC determ nat on endrobinm dennea- endrobinm num kerr. , denneanum kerr. , / 0 ,