幽门螺杆菌耐药情况分析.pdfVIP

窑医学检验 窑 中国医药导报 2014年7月第 11卷第20期 幽门螺杆菌耐药情况分析 罗昭逊 莫 非 杨国珍 张 姝 张 然 孙朝琴 袁 竺 贵阳医学院医学检验学院袁贵州贵阳 550004 [摘要] 目的调查贵阳医学院附属医院上消化道疾病患者中分离出的幽门螺杆菌渊Helicobacterpylori袁Hp冤对常用 抗生素的耐药情况袁 为临床选用抗生素治疗Hp提供实验室依据遥 方法 收集2012年 1月耀2013年 1月贵阳 医学院附属医院胃镜室患者胃黏膜标本580例袁微需氧环境培养5耀7 d袁对临床 Hp菌株采用E-test法检测对 5种常用抗生素渊甲硝唑尧阿莫西林尧克拉霉素尧左氧氟沙星尧四环素冤的耐药情况遥 结果 580例胃黏膜标本其阳 性检出率为57.6%渊334/580冤袁污染例数为21株曰经分离培养得到的313株Hp对甲硝唑尧左氧氟沙星尧四环素尧阿 莫西林及克拉霉素的耐药率分别为 87.2%尧46.9%尧23.4%尧25.5%和8.5%遥 结论 贵阳医学院附属医院幽门螺杆 菌对甲硝唑的耐药性较高袁对克拉霉素的敏感性较好遥 [关键词] 幽门螺杆菌曰抗生素曰药敏实验曰耐药性 [中图分类号] R378 [文献标识码]A [文章编号] 1673-7210渊2014冤07渊b冤-0088-03 Studyofdrugtolerance ofhelicobacter pylori LUO Zhaoxun MO Fei YANG Guozhen ZHANG Shu ZHANG Ran SUN Chaoqin YUANZhu Departmentof Laboratory, GuiyangMedical University, GuizhouProvince, Guiyang 550004, China [Abstract]ObjectiveTo investigatethe drugresistance of helicobacter pylori (Hp)separatedfrom the patients inAf鄄 filiated Hospital of Guiyang Medical University with upper gastrointestinal diseases to the common antibiotic and to provide laboratory basis for the clinical treatment of Hp with antibiotic. Methods 580 gastric mucosa samples of pa鄄 tients in the gastro-endoscope room of Affiliated Hospital of Guiyang Medical University from January 2012 to January 2013 were collected, then they were cultivated in the micro aerobic environment for 5-7 days, and the clinical drug tolerance conditions of the Hp strain to the 5 common antibiotics (Metronidazole, Amoxicillin, Clarithromycin, Lev鄄 ofloxacin andTetracycline) were inspectedwith the E-testmethod.ResultsThe positive rate ofthe 580 casesof gastric mucosa sampleswas 57.6% (334/580),the contaminated caseswere 21 strains, and the drug tolerance conditions of the 313Hp strainsafter separationand cultivationto