有性多倍化白菜四倍体雄性不育花药发育的解剖学研究.pdfVIP

ofAnhui 安徽农业科学，Journal A加．Sci．2012，40(35)：16991—16992 责任编辑刘月娟责任校对李岩 有性多倍化白菜四倍体雄性不育花药发育的解剖学研究 钟程1，田鑫2，张蜀宁2，张文华1(1．凯里学院环境与生命科学学院，贵州凯里556011；2．南京农业大学，江苏南京210095) 摘要[目的]对有性多倍化雄性不育系花药的败育时期和方式进行研究，为雄性不育基础研究提供理论依据。[方法]以同源四倍体 白菜为母本，以秋水仙素诱导筛选出含2n配子较高的二倍体为父本杂交获得四倍体不育系及其保持系为材料，采用常规石蜡切片法对 其花药进行解剖学研究，观察其花药的败育时期。[结果]退化的雄蕊可分为4种类型，均败育于孢原细胞分化期，始终处在孢原细胞 期，无绒毡层与花粉母细胞的分化，不形成药室，属孢子体败育型。I结论]有性多倍化白菜四倍体雄性不育的4种类型都是孢子体败 育型。 关键词白菜；有性多倍化；雄性不育；花药发育 中图分类号S634文献标识码A 文章编号0517—6611(2012)35—16991—02 StudiesAnther 4xSexual in on of MaleSterileLines Chinese Development Polyploidization Non-headingCabbage ZHONGetal ofEnvironmentandLife 55601 Cheng Science，Kaili 1) (CoHege University，Kaili，Guizhou on ofmale ofsexual linesin Abstract[Objective]Cytologicalinvestigationmicrosporogenesissterility polyploidizationmale．sterilenon—heading Chinese werestudiedto theoreticalbasisforthemale anatomicalstructureand wereob— cabbage provide sterility．[Method]Anther development served a slicesonthe of4xCMSsexual anditsmaintainerlineinChinese usinglightmicroscopethroughparaffin production by polyploidization of4xCMSWS atthe werefour inthe stamens．Theabortionofstamenoccurred cabbage．Abortionstage ob∞rved．[-Result]Theretypes degraded of cell whichtherewasnodifferentiationoffibrous and mothercell．It to stagearchesporialdifferentiation，at layer．tapetummicrospore belonged male ofthe4xsexual CMSlinesin Chinese non-sporangecytoplasmicsterility．[Conclusion]Fourtypes polyploidizationnon—headingcabbage were abortion．