基于转录组数据桔小实蝇微卫星位点信息分析.pdfVIP

网络出版时间：2014-04-09 09:23 网络出版地址：/kcms/doi/10.13287/j.1001-9332022.html 基于转录组数据的桔小实蝇微卫星位点信息分析*1 魏丹丹 石俊霞 张夏瑄 陈世春 魏 冬 王进军 (西南大学植物保护学院昆虫学及害虫控制工程重庆市重点实验室,重庆 400716) 摘 要 以桔小实蝇为材料,从其转录组数据库中筛选功能微卫星(EST-SSR)序列,并进行 SSR 位点的信 息分析.共获得 1890 个 EST-SSR 位点,可用于引物设计的 SSR 数为 1296 个.EST-SSR 平均分布频率为 1/10.21 kb,但这种分布频率在不同重复类型 SSR 之间相差很大.其中,三碱基重复 SSR 在该种昆虫的 EST-SSR 中出现的频率最高,结合其他文献推断三碱基重复可能是所有昆虫 EST-SSR 的优势类型.本文 共设计了 42 对桔小实蝇 EST-SSR 引物,其中有 18 对引物可以扩增得到预期大小的条带.结合相关文献 资料,探讨了基于转录组数据发掘昆虫 SSR 的前景和挑战以及进行转录组EST-SSR 筛选时应注意的问 题. 关键词 微卫星 转录组 高通量测序 分子标记 实蝇 中图分类号Q968.1 文献标识码 A Analysis of microsatellite loci from Bactrocera dorsalis based on transcriptome dataset. WEI Dan-dan, SHI Jun-xia, ZHANG Xia-xun, CHEN Shi-chun, WEI Dong, WANG Jin-jun (Chongqing Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing 400716, China) Abstract: The transcriptome database of the oriental fruit fly, Bactrocera dorsalis (Hendel), was used to identify the functional gene-microsatellite (EST-SSR) markers and to analyze the SSR loci information. In total, 1890 EST-SSR loci were identified, of which, 1296 SSR sequences could be used for primer design. The average distribution frequency of the transcriptomic SSRs was 1/10.21 kb. However, these distribution frequencies varied considerably among different types of repeat SSRs. Among of the different types of repeat SSRs, the highest frequency of tri-nucleotide repeat SSRs were found in EST-SSR of B. dorsalis