不同溶质型桃果实成熟衰老过程中线粒体内细胞色素氧化酶变化研究_阚娟_刘俊_陆燕飞.pdfVIP

网络出版时间：2014-01-08 09:02 网络出版地址：/kcms/detail/11.2206.TS0902.002.html 2014-01-03 不同溶质型桃果实成熟衰老过程中线粒 体内细胞色素氧化酶变化研究 阚 娟，刘俊，陆燕飞，金昌海* (扬州大学食品科学与工程学院，江苏 扬州 225127) 摘 要：以软溶质型桃‘雨花三号’和硬溶质型桃‘加纳岩’果实为试材，研究不同溶质型桃成熟衰老过程 中细胞色素氧化酶(COX)活性以及线粒体编码的 COX 三种亚基（COXI、COXII、COXIII ）基因表达量的变化。 结果表明：桃果实成熟前期，COX 活性不断增加，成熟后期，COX 活性开始下降。“加纳岩”COX 三种亚 基基因的表达量高于“雨花三号”且 COX 三种亚基在桃果实成熟前期表达量较高。说明线粒体 DNA 编码的 COX 三种亚基主要作用于桃果实成熟前期，为果实生长代谢提供所需能量，到了后期表达量降低，活性受影 响，导致果实线粒体内的活性氧积累，机体代谢失衡，呼吸速率增加，线粒体编码的 COX 基因与桃果实成熟 衰老之间存在一定的内在联系。 关键词：桃；线粒体；呼吸速率；细胞色素氧化酶 Changes of Mitochondrial Cytochrome c Oxidase in Two Peach Cultivars During Maturation and Senescence * KAN Juan, Liu Jun, Lu Yan-fei, JIN Chang-hai (College of Food Science and Engineering, Yangzhou University, Yangzhou 225127, China) Abstract ：Two peach (Prumus persica) cultivars ‘Yuhuasanhao’ and ‘Jianayan’ were used as materials. Activity of cytochrome c oxidase (COX) and the gene expression of mitochondrial DNA encoded subunits of cytochrome c oxidase were determined for analyzing the relationship between them and peach fruit ripening and senescene. The results showed that COX activities in the different cultivars peach increased in the early maturity (I-III) and followed by a decrease and COX activities were higher in ‘Jianayan’ peach fruit than in ‘Yuhua 3’ peach fruit. The level of expression of mitochondrial DNA encoded subunits of CO