利用Acc1基因和 trnL-F序列探讨赖草属六个物种.docVIP

利用Acc1基因和 trnL-F序列探讨赖草属六个物种 的系统关系及其母本来源 生物科学 2003级 吴莉 指导老师 周永红 教授 摘要：本研究利用质体乙酰辅酶羧化酶基因（Acc1）对赖草属六个物种（Leymus L. angustus、L racemosus、L arenarius、Ltriticoides、L ambiguus）Psathyrostachys、Pseudoroegneria、ThinopyrumLophopyrum部分物种进行了比较分析。利用叶绿体trnL-F序列构建赖草属这六个物种与小麦族19个基因二倍体属植物系统发育树，探讨赖草属六个物种的母本来源。Acc1基因分析结果表明赖草属六个物种与Psathyrostachys聚为一支，而Pseudoroegneria、Thinopyrum及Lophopyrum聚为另一支。trnL-F序列分析结果显示赖草属六个物种与Psathyrostachys形成一支，而另一支包括其它18个基因组的二倍体物种。基于Acc1基因和trnL-F序列数据，可以推测：（1）赖草属六个物种含有新麦草属的Ns基因组；（2）赖草属Xm基因组与Eb、Ee、St基因组存在较大的差异；（3）赖草属六个物种母本来源于新麦草属。关键词：赖草属，系统，质体乙酰辅酶羧化酶基因(Acc1)，叶绿体trnL-F序列Phylogenetic relationships and the maternal donor of six Leymus species based on the nuclear Acc1 gene and chloroplast trnL-F sequences WU Li Biological Science, Grade 2003 Directed by ZHOU Yong-Hong (Prof. Ph. D) Abstract: The phylogenetic relationships among six species of Leymus (L. karlinii, L. angustus, L. racemosus, L. arenarius, L. triticoides, L. ambiguous) and some species of Psathyrostach, Pseudoroegneria, Thinopyrum and Lophopyrum were investigated using the sequences of a single-copy nuclear gene encoding plastid acetyl-CoA carboxylase. To estimate the maternal donor of Leymus species, six Leymus species and monogenetic diploid representing 19 genomes were used to create phylogenetic tree based on the chloroplast trnL-F sequences. In Acc1 gene data analysis, two clades were showed. One clade included the species of Leymus and Psathyrostachys, while the other clade comprised the species of Pseudoroegneria、Thinopyrum and Lophopyrum. trnL-F sequences data, two groups were also showed. One contained Leymus and Psathyrostachys species, while the other consisted of the monogenetic diploid representing 18 genomes species. It is concluded that (1) Leymus species contains the Ns genome from Psathyrostachys species; (2) the Xm genome of Leymus is different from the Eb, Ee and St genome; (3) the maternal donor of Leymus is the species of Psathyrostachys. Keywords: L