浅刺合通窍活血汤对脑梗塞神经细胞及血浆TXB2和6-酮-PGF1a实验研究.pdfVIP

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浅刺合通窍活血汤对脑梗塞神经细胞及血浆TXB2和6-酮-PGF1a实验研究.pdf

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目录中文摘要·····················································1 英文摘要 ·····················································3 引言 ·····················································6 正文 ·····················································7 第一章实验研究 ···········································7 1.实验材料···················································7 1.1 实验动物··················································7 1.2 实验用药··················································7 1.3 实验主要试剂和主要仪器 ····································7 1.3.1 主要试剂 ··············································7 1.3.2 主要仪器 ················································7 2.实验方法 ··················································8 2.1 实验动物分组和处理方法····································8 2.2 脑梗塞大鼠模型的制备 ······································9 2.2.1 血栓子的制作 ···········································9 2.2.2 模型操作步骤 ···········································9 2.3 模型判断标准 ············································10 2.4 实验取材及观察指标······································10 2.4.1 脑组织的采集和固定 ···································10 2.4.2 血浆的采集 ···········································11 2.5 观察指标的检测方法 ······································11 2.5.1 脑组织C-jun 的检测方法 ································11 2.5.2 脑组织NGF 的检测方法 ··································12 2.5.3 血浆TXB 检测方法 ····································12 2 2.5.4 血浆 6-酮-PGF1a 检测方法 ······························13 1 2.6 统计学处理方法 ·········································13 3.实验结果··················································13 3.1各组大鼠神经功能评分比较 ·································13 3.2 苏木素-伊红染色（HE 染色）································14 3.3 三个时间点各组大鼠脑组织C-jun 蛋白表达比较 ···············14 3.4 三个时间点各组大鼠脑组织NGF 表达比较 ····················15 3.5三个时间点各组大鼠血浆TXB 的比较 ························15 2 3.6 三个时间点各组大鼠血浆6-酮-PGF1a 的