Systemic Signaling and Local Sensing of Phosphate in Common Bean: Cross-Talk between Photosynthate and MicroRNA399.pdfVIP
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Systemic Signaling and Local Sensing of Phosphate in Common Bean: Cross-Talk between Photosynthate and MicroRNA399.pdf
MolecularPlant ·Volume3 ·Number2 ·Pages428-437 ·March2010 RESEARCHARTICLE
SystemicSignalingandLocalSensing
ofPhosphateinCommonBean:Cross--Talk
betweenPhOtOsynthateandMicroRNA399
Jun-QiLiu。一-,DeborahL.Allan andCarrollRVance。,‘
aDepartmentofAgronomyandPlantGenetics,UniversityofMinnesota,StPaul,MN55108,USA
bDepartmentofSoil,WaterandClimate,UniversityofMinnesota,StPaul,MN 55108,USA
cUnitedStatesDepartmentofAgriculture,AgriculturalResearchService,StPaul,MN 55108,USA
ABSTRACT Shoot-to-rootcommunicationiscrucialforplantacclimationtophosphorus(P)-deficiency.Bothsugarsand
miRNAshavebeenimplicatedaspotentiaIsignalmoleculestransportedthroughphloem from shoottorootforthereg.
ulationofgeneexpressionandPiuptakeintheroot.Bystudyingtheexpressionpatternsofbothaserine/threoninephos-
phatasegene(PvHAD1)andmicroRNA399(miR399)incommonbean(PhaseolusvulgarisL.),weprovideevidenceforthe
interactionbetw eenlight,phloem transport,andmiR399inthesystemicregulationofgeneexpressionunderP-deficiency.
Especially,miR399expressioninboththeshootandtherootrequiresphotosyntheticcarbonassimilationduringtheonset
ofP-deficiency.Incontrasttosystemicsignaling,localsensingwastheprimarycausalfactorforrapiddown-regulationof
PvHAD1byPipriortothereductionofmiR399leveIinP-deficientroots.Furthermore.thisinitiaIresponsetoPiinP.de.
ficientrootwasalsomimickedbythePianalog.phosphonate(Phi).Ourcurrentfindingssuggestthatplantshavedevel—
opedahighlycoordinateddualregulatorypathway,namelylong-distancesignalingofP-deficiencyfrom shoottoroot
versusIocaIsensingofPiintheroot.
Keywords: Roots;nutrientstress;phOtOsynthate;signaling.
INTRODUCTlON
playsanimportantroleinphosphate(Pi)homeostasisinArabi-
Whiletheprimarysourceforcarbon(C)inhigherplantsisfrom dopsisandrice(Fujiieta1.,2005;Sunkareta1.,2005;Chioueta1.,
ph0t0syntheticcarbonassim_lationintheshoot,theuptakeof
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