6种苯系物对等鞭金藻和新月菱形藻的生长抑制.docVIP

6种苯系物对球等鞭金藻和新月菱形藻的生长抑制王摆1高士博12，董颖1，周遵春1 1. 辽宁省海洋水产科学研究院，大连 116023 2. 大连海洋大学，大连 116023 收稿日期：2013-04-10 录用日期：2013-06-25 摘要：海洋环境中苯系物污染主要来源于海洋溢油事故以及沿海石油化工企业的废水排放。为探究苯系物对海洋微藻的毒性作用，选择球等鞭金藻和新月菱形藻作为受试生物，分别考察了苯、甲苯、乙基苯、邻–二甲苯、间–二甲苯和对–二甲苯6种苯系物对2种海洋微藻生长的影响。结果表明，在0.25～ mg·L-1暴露浓度下，6种苯系物对2种微藻生长具有显著的抑制作用，随着暴露浓度的升高，抑制作用明显增强。苯、甲苯、乙基苯、邻–二甲苯、间–二甲苯、对–二甲苯对球等鞭金藻的24 h的半数效应浓度(24 h–EC50)分别为：17.07、12.88、7.58、0.55、0.36、0.27 mg·L-1；对新月菱形藻的24 h–EC50值分别为：1.03、0.68、0.46、0.40、0.42、0.38 mg·L-1。上述研究结果为确定苯系物海洋环境质量标准、保护海洋生态环境提供了基础数据。 关键词：苯系物；球等鞭金藻；新月菱形藻；生长抑制 文章编号： 中图分类号： 文献标识码：A Inhibitory Effect of Six Kinds of BTEXs on Growth of the Chrysophyceae Isochrysis galbana and the Diatom Nitzschia closterium Wang Bai1, Gao Shibo1,2, Dong Ying1, Wang Xiaoyue1, Zhou Zunchun1,* 1. Liaoning Ocean and Fisheries Science Research Institute, Dalian 116023, China 2. Dalian Ocean University, Dalian 116023, China Received 10 April 2013 Accepted 25 June 2013 Abstract: The marine pollution of benzene series (BTEXs), including benzene, toluene, ethyl benzene and xylene, is mainly from the marine oil spill and coastal petrochemical wastewater discharge. In order to reveal the toxic effect of BTEXs on marine microalgae, the chrysophyceae Isochrysis galbana and the diatom Nitzschia closterium were exposed to benzene, toluene, ethyl benzene, o–xylene, m–xylene and p–xylene, and the their growth rates were detected. The results showed that the growth of I. galbana and N. closterium were inhibited dramatically by the BTEXs at the exposed concentration from 0.25 to 64.0 mg·L-1. The growth inhibition was enhanced with the concentration of BTEXs in well dose-effect manner. The 24 h-EC50 of benzene, toluene, ethyl benzene, o–xylene, m–xylene and 基金项目：海洋 (201205012-7)；大连市科学技术基金(2012J21DW029) 作者简介：王摆(1981)，男，助理研究员，研究方向海洋生态毒理学E-mail: wangbai1980@；通讯作者E-mail: zunchunz@ p–xylene to I. galbana was 17.07, 12.88, 7.58, 0.55, 0.36 and 0.27 mg·L-1. The 24 h-EC50 of the correspo