Fas 和FasL 在急性胰腺炎中的共表达及其与凋亡的关系 李震东.docVIP

Fas和FasL在急性胰腺炎中的共表达及其与凋亡的关系 作者：李震东1；马清涌1；徐军1；李明2　　(西安交通大学1第一医院肝胆外科，2医学院组胚教研室，陕西??西安??710061) 摘要：目的??探讨凋亡调控基因Fas、FasL在大鼠急性胰腺炎中的表达及其与腺泡细胞凋亡的关系。方法??通过胰胆管内逆行注射不同浓度的牛磺胆酸钠，建立不同严重程度的急性胰腺炎模型，用RT-PCR、免疫组化法检测胰腺组织Fas、FasL mRNA及蛋白的表达，原位末端标记法检测各组大鼠胰腺腺泡细胞凋亡。结果??在正常的胰腺腺泡细胞中即存在着Fas、FasL mRNA及蛋白的表达，且呈现共区域化特点，凋亡细胞极少。在炎症程度较轻的胰腺组织，Fas和FasL mRNA的表达水平显著提高，腺泡细胞凋亡指数亦明显提高，随胰腺炎症程度的加重，Fas和FasL mRNA的表达逐渐下降，腺泡细胞凋亡指数亦逐渐下降。结论??Fas/FasL系统可能参与了正常胰腺组织的细胞更新和自稳；是介导急性胰腺炎时腺泡细胞凋亡的一个重要途径。 关键词：急性胰腺炎；细胞凋亡；Fas；FasL 中图分类号：R657.5??文献标识码：A??文章编号：1673-4254(2006)01-0025-05 Colocalized expression of Fas and Fas-Ligand in acute pancreatitis and its correlation with cell apoptosisLI Zhen-dong1；MA Qing-yong1；XU Jun1；LI Ming2Department of Hepatobiliary Surgery, First Hospital of Xi’an Jiaotong University1, Department of Histoembryology, Medical College2, Xi’an Jiaotong University, Xi’an 710061, ChinaAbstract: Objective To study the expressions of Fas and Fas-Ligand (FasL) genes in rats with acute pancreatitis (AP) and their relation to apoptosis of pancreatic acinar cells. Methods Rat models of acute pancreatitis with varied severity were established by retrograde injection of sodium taurocholate at different concentrations via the apancreatobiliary duct. The expressions of Fas and FasL mRNAs and proteins were detected by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. The apoptosis of acinar cells was quantified by in situ nick ending-labeling technique. Results of expressions fas, FasL mRNA and protein were colocalized in normal pancreatic acinar cells. In pancreatic tissue with less severe inflammation, the expressions of Fas and FasL mRNA increased significantly, and the apoptosis index of the acinar cells was also elevated; with the increase in the severity of the pancreatitis, the expression of Fas and FasL mRNAs were gradually lowered and apoptosis index of the acinar cells was increased. Conclusion??Fas/FasL system might be involved in the renewal and homeostasis of n